Neuropeptide Techniques / edited by Illana Gozes.

Format:

Book

Contributor:

Gozes, Illana, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 39.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 39

Language:

English

Subjects (All):

Neurosciences.

Proteomics.

Biochemistry.

Human physiology.

Neurobiology.

Protein Science.

Human Physiology.

Local Subjects:

Neurosciences.

Proteomics.

Protein Science.

Human Physiology.

Neurobiology.

Physical Description:

1 online resource (XII, 160 pages) : 52 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2008.

System Details:

text file PDF

Summary:

Major advances in molecular biology, alternative RNA splicing, protein processing, identification of gene control elements, transgenic animals and bioinformatics open up multiple research avenues and allow for better understanding of neuropeptide production and function. With a selected description of techniques in neuropeptide research, Neuropeptide Methods covers aspects of design, synthesis and biological evaluation of peptides and peptide analogues. Written by leaders in the field, this book explains primary cell cultures and cell lines, as well as techniques in neuropeptide processing, trafficking and secretion. In vivo techniques quantifying blood-brain barrier permeability to small proteins in mice and developmental milestones of behavior in newborn mice are described. Neuropeptide Methods provides the reader with broad perspectives and breadth of knowledge on current topics related to neuropeptide research.

Contents:

A Short Introduction

Preface

Contributors

The Design, Synthesis, and Biological Evaluation of VIP and VIP Analogs David Dangoor, Sara Rubinraut, Mati Fridkin, and Illana Gozes

Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example Illana Gozes, Miri Holtser-Cochav, and Karin Vered

Primary Cell Cultures and Cell Lines Inna Divinski, Inbar Pilzer, and Illana Gozes

Transfection of DNA into Cells Inbar Pilzer, Inna Divinski, and Illana Gozes

Transgenic Delivery and Detection of GFP in Neuropeptide Neurons J.L. Holter, J.S. Davies, P.-S. Man, T. Wells, and D.A. Carter

Integrative Technologies for Neuropeptide Characterization Sergey E. Ilyin and Carlos R. Plata-Salamán

Techniques in Neuropeptide Processing, Trafficking, and Secretion Niamh X. Cawley, Tulin Yanik, Irina Arnaoutova,Hong Lou, Nimesh Patel, and Y. Peng Loh

In vivo Techniques Quantifying Blood-Brain Barrier Permeability to Small Proteins in Mice Weihong Pan, and Abba J. Kastin

Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs Terry W. Moody, Michael Schumann, and Robert T. Jensen

Developmental Milestones in the Newborn Mouse Joanna M. Hill, Maria A. Lim, and Madeleine M. Stone.

Other Format:

Printed edition:

ISBN:

9781603270991

Access Restriction:

Restricted for use by site license.