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Developmental Biology of the Sea Urchin and Other Marine Invertebrates : Methods and Protocols / edited by David J. Carroll, Stephen A. Stricker.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Carroll, David J., editor.
Stricker, Stephen A., editor.
SpringerLink (Online service)
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1128.
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1128
Language:
English
Subjects (All):
Life sciences.
Cytology.
Developmental biology.
Animal models in research.
Life Sciences.
Cell Biology.
Developmental Biology.
Animal Models.
Local Subjects:
Life Sciences.
Cell Biology.
Developmental Biology.
Animal Models.
Physical Description:
1 online resource (XIV, 347 pages) : 69 illustrations, 14 illustrations in color.
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.
System Details:
text file PDF
Summary:
In Developmental Biology of the Sea Urchin and Other Marine Invertebrates: Methods and Protocols, expert researchers in the field detail many of the methods which are now used to study sea urchins and other marine invertebrates in the laboratory. These include methods and protocols on imaging, other useful experimental tools for cell, developmental biology research, variety of molecular biological methods, and strategies for utilizing the sea urchin genome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Developmental Biology of the Sea Urchin and other Marine Invertebrates: Methods and Protocols seeks to aid scientists in the further study into sea urchins and other marine invertebrates.
Contents:
Culturing Larvae of Marine Invertebrates
Obtaining Gametes and Embryos of Ascidians
Artificial Seawater Culture of the Gastropod Crepidula fornicata for Studies of Larval Settlement and Metamorphosis
Trichoplax adhaerens, an Enigmatic Basal Metazoan with Potential
Rearing Gymnolaemate Bryozoan Larvae for Cellular and Molecular Analyses
An Invertebrate Embryologist's Guide to Routine Processing of Confocal Images
TEM Analyses of Chaetognath Reproductive Organs
SEM Analysis of Marine Invertebrate Gametes
Imaging Neural Development in Embryonic and Larval Sea Urchins
Preparation and Use of Sea Urchin Egg Homogenates
Microinjection and 4D Fluorescence Imaging in the Eggs and Embryos of the Ascidian Phallusia mammillata
Isolating Specific Embryonic Cells of the Sea Urchin by FACS
Biotinylation of Oocyte Cell Surface Proteins of the Starfish Patiria miniata
Perturbations to the Hedgehog Pathway in Sea Urchin Embryos
Regulation of DNA Synthesis at the First Cell Cycle in the Sea Urchin in vivo
Immunoblotting Analyses of Changes in Protein Phosphorylations During Oocyte
Maturation in Marine Nemertean Worms
Multi-color Labeling in Developmental Gene Regulatory Network Analysis
A 96-well Plate Format for Detection of Marine Zooplankton with the Sandwich Hybridization Assay
Isolation and Assessment of Signaling Proteins from Synchronized Cultures During Egg Activation and Through the Egg-to-Embryo Transition in Sea Urchines
Tools for Sea Urchin Genomic Analysis
Antibody Inhibition of Protein Activity in Starfish Oocytes
Immunophotoaffinity Labeling of the Binding Proteins for 1-Methyladenine, an Oocyte-maturation Inducing Hormone of Starfish. .
Other Format:
Printed edition:
ISBN:
9781627039741
Access Restriction:
Restricted for use by site license.

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