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Stem Cells and Aging : Methods and Protocols / edited by Kursad Turksen.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Turksen, Kursad, editor.
SpringerLink (Online service)
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 976.
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 976
Language:
English
Subjects (All):
Life sciences.
Cytology.
Stem cells.
Regenerative medicine.
Tissue engineering.
Life Sciences.
Cell Biology.
Stem Cells.
Regenerative Medicine/Tissue Engineering.
Local Subjects:
Life Sciences.
Cell Biology.
Stem Cells.
Regenerative Medicine/Tissue Engineering.
Physical Description:
1 online resource (XI, 179 pages) : 30 illustrations, 21 illustrations in color.
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.
System Details:
text file PDF
Summary:
Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.
Contents:
Comprehensive Hematopoietic Stem Cell Isolation Methods
Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo
Mouse Hematopoietic Stem Cell Transplantation
Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells
Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces
Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy
Cardiac Stem Cell Senescence
Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions
Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells
Intra-Femoral Injection of Human Mesenchymal Stem Cells
Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency
Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging
Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue.
Other Format:
Printed edition:
ISBN:
9781627033176
Access Restriction:
Restricted for use by site license.

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