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MicroRNAs in Development : Methods and Protocols / edited by Tamas Dalmay.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 732.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 732
- Language:
- English
- Subjects (All):
- Life sciences.
- Gene expression.
- Cytology.
- Life Sciences.
- Cell Biology.
- Gene Expression.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Gene Expression.
- Physical Description:
- 1 online resource (X, 200 pages) : 36 illustrations, 2 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2011.
- System Details:
- text file PDF
- Summary:
- Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.
- Contents:
- In situ Detection of microRNAs in Animals
- Detection of microRNAs in Plants by In situ Hybridization
- Detecting Animal and Plant microRNAs by Northern Blotting
- Profiling microRNAs by Real-time PCR
- Detection of Small RNAs and microRNAs using Deep Sequencing Technology
- Detection of microRNAs in Prostate Cancer Cells by microRNA Array
- microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture
- Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila
- Engineering Elements for Gene Silencing: The Artificial microRNAs Technology
- Mimicry Technology: Suppressing Small RNA Activity in Plants
- Experimental Validation of MicroRNA Targets using a Luciferase Reporter System
- Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes
- Comprehensive Identification of miRNA Target Sites in Live Animals
- Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes.
- Other Format:
- Printed edition:
- ISBN:
- 9781617790836
- Access Restriction:
- Restricted for use by site license.
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