Human Retroviruses : Methods and Protocols / edited by Elisa Vicenzi, Guido Poli.

Format:

Book

Contributor:

Vicenzi, Elisa, editor.

Poli, Guido, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1087.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1087

Language:

English

Subjects (All):

Medicine.

Immunology.

Virology.

Biomedicine.

Local Subjects:

Biomedicine.

Immunology.

Virology.

Physical Description:

1 online resource (XVIII, 345 pages) : 62 illustrations, 38 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.

System Details:

text file PDF

Summary:

Human Retroviruses: Methods and Protocols collects key experimental protocols that have provided the basis of the major discoveries of the field. Split into five sections, this detailed volume covers mapping of the HIV life cycle, isolation, co-receptor use, and cell tropism of HIV-1, in vivo quantification of HIV-1, biological aspects of HIV-1, as well as HTLVs. Some articles explore "assay and function of accessory genes", largely involving the interface between retroviral and host factors, the extracellular role of Tat and Tax, resembling the function of cytokines, and the biotechnological exploitation of HIV as lentiviral vector to carry foreign genes with therapeutic value. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Human Retroviruses: Methods and Protocols provides state-of-art methodological protocols from world leaders in human retrovirology, essential for any lab working this vital field.

Contents:

Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand Binding Assay

CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5α

The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating

The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells

Imaging HIV-1 Nuclear Pre-Integration Complexes

HIV-1 Reverse Transcription

RNase H: Specificity, Mechanisms of Action, and Antiviral Target

HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat

HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export

HIV-1 Accessory Proteins: Nef

HIV-1 Accessory Proteins: VpR

HIV-1 Accessory Proteins: Vpu and Vif

SIVSM/HIV-2 Vpx Proteins : Function and Uses in the Infection of Primary Myeloid Cells

Imaging of HIV Assembly and Release

HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells

Determination of HIV-1 Co-Receptor Usage

The Macrophage and HIV: Basic Concepts and Methodologies

HIV Infection of Dendritic Cells

Histocultures (Tissue Explants) in Human Retrovirology

Single Copy Quantification of HIV-1 in Clinical Samples

Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells

HIV-1 Based Lentiviral Vectors

Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone

Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques

Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR

Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real Time RT-PCR (qRT-PCR).

Other Format:

Printed edition:

ISBN:

9781627036702

Access Restriction:

Restricted for use by site license.