Single Molecule Enzymology : Methods and Protocols / edited by Gregory I. Mashanov, Christopher Batters.

Format:

Book

Contributor:

Mashanov, Gregory I., editor.

Batters, Christopher, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 778.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 778

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Enzymology.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Enzymology.

Physical Description:

1 online resource (XI, 294 pages) : 88 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.

Contents:

Single Enzyme Studies: A Historical Perspective

Functional Surface-Attachment in a Sandwich Geometry of GFP-Labeled Motor Proteins

Studying Kinesin's Enzymatic Cycle Using a Single-Motor Confocal Motility Assay, Employing Förster Resonance Energy Transfer

Fluorescence Imaging with One Nanometer Accuracy: In Vitro and In Vivo Studies of Molecular Motors

Snapshots of Kinesin Motors on Microtubule Tracks

Structural and Dynamic Characterization of Biochemical Processes by Atomic Force Microscopy

Using Optical Tweezers to Study Fine Details of Myosin ATPase Mechanochemical Cycle

Quantum Dot Labeling Strategies to Characterize Single Molecular Motors

Imaging Individual Myosin Molecules Within Living Cells

Single Molecule Measurements Using Microneedles

Fluorescent Nucleoside Triphosphates for Single Molecule Enzymology

Probing the Mechanics of the Complete DNA Transcription Cycle in Real-Time Using Optical Tweezers

A Single-Molecule Approach to Visualize the Unwinding Activity of DNA Helicases

Real-Time Single-Molecule Observation of Green Fluorescent Protein Synthesis by Immobilized Ribosomes

Single-Molecule Measurements of Topoisomerase Activity with Magnetic Tweezers

Assembly of Recombinant Nucleosomes on Nanofabricated DNA Curtains for Single Molecule Imaging

Simultaneous Observation of Chemomechanical Coupling of a Molecular Motor

Microsecond Resolution of Single Molecule Rotation Catalyzed by Molecular Motors.

Other Format:

Printed edition:

ISBN:

9781617792618

Access Restriction:

Restricted for use by site license.