Androgen Action : Methods and Protocols / edited by Fahri Saatcioglu.

Format:

Book

Contributor:

Saatcioglu, Fahri, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 776.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 776

Language:

English

Subjects (All):

Medicine.

Molecular biology.

Endocrinology.

Biomedicine.

Molecular Medicine.

Local Subjects:

Biomedicine.

Molecular Medicine.

Endocrinology.

Physical Description:

1 online resource (XI, 386 pages) : 68 illustrations, 12 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

Androgens play a critical role in the development and maintenance of the male reproductive system and affect important physiological processes and pathological conditions, including the homeostasis of the normal prostate and prostate cancer. Androgen Action: Methods and Protocols is designed to provide a tool box to study various phases of androgen action, from its entry to the cell to the phenotypic response that the cell mounts, with up-to-date techniques for biochemists, molecular biologists, cell biologists, geneticists, and pathologists. The volume opens with a brief review of the research history on androgen action and prostate carcinogenesis, followed by chapters that cover state-of-the-art methods to determine androgen levels in biological tissues and fluids, experimental procedures to study the various aspects of androgen receptor activity, and methodology to study salient examples of interactions between androgen signalling and other major signalling pathways in cells. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Androgen Action: Methods and Protocols provides a comprehensive overview of, and practical guidance on, the diverse methodologies that are propelling androgen action research forward, both for normal physiology as well as in disease states.

Contents:

Molecular Mechanisms of Androgen Action - A Historical Perspective

Androgen Action during Prostate Carcinogenesis

Androgen Quantitation in Prostate Cancer Tissue using Liquid Chromatography Tandem Mass Spectrometry

Ligand Competition Binding Assay for the Androgen Receptor

Analysis of Androgen Receptor Activity by Reporter Gene Assays

Identification and Characterization of Androgen Response Elements

In vitro and In vivo Silencing of the Androgen Receptor

Analysis of Interdomain Interactions of the Androgen Receptor

Methods to Study Dynamic Interaction of Androgen Receptor with Chromatin in Living Cells

FRET Analysis of Androgen Receptor Structure and Biochemistry in Living Cells

Analysis of Nuclear Receptor Acetylation

Analysis of Androgen Receptor SUMOylation

Analysis of Ligand-specific Co-repressor Binding to the Androgen Receptor

Detection of Ligand-selective Interactions of the Human Androgen Receptor by SELDI-MS-TOF

Global Identification of Androgen Response Elements

Tissue Specific Knockout of Androgen Receptor in Mice

Methodology to Investigate Androgen-sensitive and Castration-resistant Human Prostate Cancer Xenografts in Preclinical Setting

Automated Microscopy and Image Analysis for Androgen Receptor Function

Androgen Regulation of ETS-gene Fusion Transcripts in Prostate Cancer

Regulation of Apoptosis by Androgens in Prostate Cancer Cells

Analysis of Androgen Receptor Rapid Actions in Cellular Signaling Pathways: Receptor /Src Association

Analysis of Androgen-induced Increase in Lipid Accumulation in Prostate Cancer Cells.

Other Format:

Printed edition:

ISBN:

9781617792434

Access Restriction:

Restricted for use by site license.