Transmembrane Signaling Protocols / edited by Dafna Bar-Sagi.

Format:

Book

Contributor:

Bar-Sagi, Dafna, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 84.

Springer Protocols (Springer-12345)

Methods In Molecular Biology™, 1064-3745 ; 84

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XIII, 328 pages) : 47 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

Transmembrane Signaling Protocols brings together a collection of practical, cutting-edge techniques for the study of cell signaling. Written by acknowledged leaders in the field, the protocols provide detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the latest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. The time-tested techniques collected in Transmembrane Signaling Protocols make available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. The book is destined to become a standard resource for both conceptual information and the practical detail needed to carry out and interpret laboratory procedures in this dramatically advancing field.

Contents:

Overviews

Peptide Recognition Mechanisms of Eukaryotic Signaling Modules

Protein-Protein Interactions in Signaling Cascades

Transmembrane Signaling by Receptor Oligomerization

Specific Topics

Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases

Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries

Selective Antagonism of Receptor Signaling Using Antisense RNA to Deplete G-Protein Subunits

Microinjection of Antisense Oligonucleotides and Electrophysiological Recording of Whole-Cell Currents as Tools to identify Specific G-Protein Subtypes Coupling Hormone Receptors to Voltage-Gated Calcium Channels

Oocytes Microinjection Assay to Study the MAP-Kinase Cascade

Mammalian Cell Microinjection Assay to Study the Function of Rac and Rho

Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS

Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade

Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipases C and D

Two-Hybrid Analysis of Ras-Raf Interactions

Cloning and Mutational Analysis of the She-Phosphotyrosine Interaction/ Phosphotyrosine-Binding Domain

Cloning and Mutational Analysis of the Shc-Phosphotyrosine Interaction/Phosphotyrosine-Binding Domain

Use of Fluorescence Spectroscopy to Study the Regulation of Small G Proteins

Prenylation Assays for Small GTPases

Analysis of Myristoylated and Palmitoylated Src Family Proteins

Ultracentrifugation Technique for Measuring the Binding of Peptides and Proteins to Sucrose-Loaded Phospholipid Vesicles

Biochemical and Biological Analyses of Farnesyl-Protein Transferase Inhibitors

Identification and Characterization of Small GTPase-Associated Kinases

Functional Studies of Dual-Specificity Phosphatases

Functional Studies of Dual-Specificity Phosphatases.

Other Format:

Printed edition:

ISBN:

9781592595686

Access Restriction:

Restricted for use by site license.