Protocols in Molecular Parasitology / edited by John E. Hyde.

Format:

Book

Contributor:

Hyde, John E., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 21.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 21

Language:

English

Subjects (All):

Life sciences.

Microbiology.

Life Sciences.

Local Subjects:

Life Sciences.

Microbiology.

Physical Description:

1 online resource (XIV, 470 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1993.

System Details:

text file PDF

Summary:

Protocols in Molecular Parasitology offers a wide range of experi- mental protocols, each written by experts, to research workers interested in exploring the molecular aspects of parasitology. This is a field that has expanded enormously in scope and potential over the last decade, although it is as yet significantly underrepresented in the literature out- side of academic journals. The need for manuals such as this one stems in part from the increasing difficulty of reproducing experimental tech- niques from reports in standard journal papers, where pressures on space usually result in highly abbreviated descriptions. The "Notes" sections of the following chapters are particularly aimed at counteracting this problem. Since the emphasis of the book is on aspects of molecular analysis, it was possible to devote only a limited amount of space to describing protocols for culture and isolation of the primary parasitic material. Therefore, of the numerous parasitic systems that are currently being investigated in laboratories around the world, only techniques for c- turing the "big five" are described here, since they collectively account for the great bulk of research in this field. It is also possible that some minor front-end modifications to certain protocols may be necessary, depending on exactly which parasite is being studied.

Contents:

Culturing and Biological Cloning of Trypanosoma brucei

Culturing and Biological Cloning of Trypanosoma cruzi

In Vitro Cultivation and Biological Cloning of Leishmania

Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages)

Synchronization and Cloning of Malaria Parasites

The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies

The Culture of Schistosoma mansoni and Production of Life Cycle Stages

Preparation of DNA and RNA from Trypanosoma brucei

Isolation of RNA and DNA from Trypanosoma cruzi

Isolation of DNA and RNA from Leishmania

The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum

Extraction and Purification of Nucleic Acids from Schistosomes

Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting

The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification

Analysis of the DNA of Parasitic Protozoa by Flow Cytometry

PCR orLowLevel Detection of Malaria Parasites in Blood

PCR Methods for Identification of Point Mutations and Gene Rearrangements

Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques

Ribosomal RNA Probes for Detection and Identification of Species

Rapid Sequencing of Parasite rRNA

Antibody Screening of Expression Libraries

Antibody Select Procedure for Characterization of Expression Clones

Isolation of Parasite Genes Using Synthetic Oligonucleotides

Chromosome Mapping Methods for Parasitic Protozoa

Transfection ofLeishmania and Trypanosoma brucei by Electroporation

Two-Dimensional Polyacrylamide Gel Electrophoresis

Isoenzyme Electrophoresis for Parasite Characterization

The Surface Labeling of Schistosomes

Monoclonal Antibody Affinity Chromatography

Immunoblotting and Enzyme-Linked Immunosorbent Assay

Immunofluorescence of Parasites

Immunoelectron Microscopic Localization of Antigens in Malaria Parasites

Preparation of Blotted Membrane for Protein Microsequencing

Identification of T-Cell Epitopes.

Other Format:

Printed edition:

ISBN:

9781592595082

Access Restriction:

Restricted for use by site license.