Leukemia : Methods and Protocols / edited by Chi Wai Eric So.

Format:

Book

Contributor:

So, Chi Wai Eric, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 538.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 538

Language:

English

Subjects (All):

Medicine.

Cancer--Research.

Cancer.

Diagnosis, Laboratory.

Oncology.

Biomedicine.

Cancer Research.

Laboratory Medicine.

Local Subjects:

Biomedicine.

Cancer Research.

Oncology.

Laboratory Medicine.

Physical Description:

1 online resource (XI, 428 pages) : 87 illustrations, 12 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2009.

System Details:

text file PDF

Summary:

Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease. In Leukemia: Methods and Protocols, expert researchers bring together a wide range of state-of-the-art laboratory methods and detailed protocols that are useful for both clinical and basic research scientists working on the disease. The volume provides techniques for prenatal backtracking of leukemic clone, molecular diagnosis, detection of genome-wide genetic abnormalities and profiling, identification of unknown fusion genes, monitoring of minimal residual diseases, disease modeling using murine and human primary hematopoietic cells, studying of normal and malignant hematopoiesis, identification of interacting partners with leukemia associated oncoproteins, and global characterization of genome-wide epigenetic changes in leukemic cells. Written in the highly successful Methods in Molecular Biology™ series format, the convenient chapters contain brief introductions, lists of the necessary materials, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Leukemia: Methods and Protocols will help researchers to advance knowledge and have a better understanding of the disease, which will ultimately facilitate development of anti-cancer therapy and improve quality of life for patients.

Contents:

An Overview: From Discovery of Candidate Mutations to Disease Modeling and Transformation Mechanisms of Acute Leukemia

Backtracking of Leukemic Clones to Birth

Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia

Specialized Fluorescence In Situ Hybridization (FISH) Techniques for Leukaemia Research

LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene

Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences

MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR

Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome

Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer

Retroviral/Lentiviral Transduction and Transformation Assay

Gene Expression Profiling of Leukemia Stem Cells

Humanized Model to Study Leukemic Stem Cells

Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells via Retroviral Transduction

Ex Vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human Acute Myeloid Leukemia Stem Cells

Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry

In Vitro Differentiation of Embryonic Stem Cells as a Model of Early Hematopoietic Development

Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche

Identification of Protein Interaction Partners by the Yeast Two-Hybrid System

Complete Array of HOX Gene Expression by RQ-PCR

Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias

Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated Proteins.

Other Format:

Printed edition:

ISBN:

9781597454186

Access Restriction:

Restricted for use by site license.