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Melanoma Techniques and Protocols : Molecular Diagnosis, Treatment, and Monitoring / edited by Brian J. Nickoloff, Leroy Hood.

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Format:
Book
Contributor:
Nickoloff, Brian J., 1953- editor.
Hood, Leroy, editor.
SpringerLink (Online service)
Series:
Methods in molecular medicine 1543-1894 ; 61.
Springer Protocols (Springer-12345)
Methods in Molecular Medicine, 1543-1894 ; 61
Language:
English
Subjects (All):
Medicine.
Oncology.
Medicine & Public Health.
Local Subjects:
Medicine & Public Health.
Oncology.
Physical Description:
1 online resource (XVI, 368 pages).
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press, 2001.
System Details:
text file PDF
Summary:
With the completion of the Human Genome Project, it is now possible to decipher the biochemical cascade of events that lead to melanoma, a virulent form of cancer that is currently at epidemic proportions worldwide. In Melanoma Techniques and Protocols: Molecular Diagnosis, Treatment, and Monitoring, a broadly diverse group of researchers and clinicians offer not only reviews of the most important recent advances in melanoma oncology, tumor immunology, and pathology, but also state-of-the-art molecular techniques for probing melanoma's basic biology. These readily reproducible methods can be used to develop diagnostic approaches, therapeutics, and status monitoring of patients suffering both early- and late-stage melanoma. With emphasis on the experimental details critical to success, each method is described in step-by-step fashion by hands-on masters, often those who have developed them in their own laboratories. Notes about potential pitfalls and troubleshooting tips on how to adapt procedures to new experimental systems are also provided. Comprehensive and highly instructive, Melanoma Techniques and Protocols: Molecular Diagnosis, Treatment, and Monitoring offers clinicians and basic scientists concerned with malignant melanoma both rich new clinical perspectives and the wide ranging experimental protocols essential for achieving more rapid breakthroughs in this near-epidemic disease.
Contents:
Biology
The Many Molecular Mysteries of Melanoma
Isolation of Tumor Suppressor Genes in Melanoma by cDNA Microarray
Molecular Characterization of Melanoma-Derived Antigens
Experimental Induction of Human Atypical Melanocytic Lesions and Melanoma in Ultraviolet-Irradiated Human Skin Grafted to Immunodeficient Mice
Dissection of Immunosuppressive Effects of Ultraviolet Radiation
Antisense Oligonucleotides as Research Tools
Diagnosis
Genetic Testing in Familial Melanoma
Role of Molecular Biology in Diagnostic Pathology of Melanoma
Identification of Altered Gene Expression Associated with Pigmentary Lesions by Differential Display Analysis
Fluorescence In Situ Hybridization as a Tool in Molecular Diagnostics of Melanoma
Treatment
Antigen-Pulsed Dendritic Cell Approach to Melanoma
Gene Therapy in Melanoma
Use of Gene Gun for Genetic Immunotherapy
Role of Cytokines in Pathology of Melanoma and Use of Biologic Response Modifiers in Melanoma
Surgical Techniques for Treatment of Primary Cutaneous Melanoma
Monitoring
Utility of Fine-Needle Aspiration Biopsy for Prospective Analysis of Patients Undergoing Therapy for Metastatic Melanoma
A Molecular Technique Useful in the Detection of Occult Metastases in Patients with Melanoma
Immunoscope Analysis of T-Lymphocytes Infiltrating Melanocytic Tumors
T-Cell Receptor Clonotype Mapping Using Denaturing Gradient Gel Electrophoresis
Methods for Use of Peptide-MHCTetramers in Tumor Immunology.
Other Format:
Printed edition:
ISBN:
9781592591459
Access Restriction:
Restricted for use by site license.

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