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Calcium Signaling Protocols / edited by David G. Lambert.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 114.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 114
- Language:
- English
- Subjects (All):
- Life sciences.
- Biochemistry.
- Life Sciences.
- Biochemistry, general.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Physical Description:
- 1 online resource (XIII, 359 pages) : 63 illustrations, 2 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 1999.
- System Details:
- text file PDF
- Summary:
- In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents.
- Contents:
- General
- Fluorescent Measurement of [Ca2+]c
- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
- Specialist Measurement Systems
- Confocal Microscopy
- Measurement of Intracellular Calcium Concentration Using Confocal Microscopy
- Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i)
- Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR)
- Nonelectrophysiological measurement of Ca2+ channel activity
- Measurement of Ca2+ Entry Using 45Ca2+
- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding
- Measurement of Ins(1,4,5)P3 and Ca2+ Release from Intracellular Stores
- Measurement of Inositol (Poly)phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells
- Measurement of Inositol(1,4,5)trisphosphate Using a Stereospecific Radioreceptor Mass Assay
- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay
- Microinjection of myo-Inositol(1,4,5)trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells
- Photolysis of Caged Calcium Using a Low-Cost Flash Unit
- Measurement of Ca2+ Flux Through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology)
- Continuous Fluorescent Monitoring of Cellular Calcium Fluxe
- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
- Specialist Measurement Techniques
- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
- Measurement of Calcium and Movement in Heart Cells
- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
- Measurement of Cytosolic-Free Ca2+ in Plant Tissue
- Ca2+ Sensitive Targets
- Assay and Purification of Calmodulin-Dependent Protein Kinase
- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).
- Other Format:
- Printed edition:
- ISBN:
- 9781592592500
- Access Restriction:
- Restricted for use by site license.
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