Gene Regulatory Networks : Methods and Protocols / edited by Bart Deplancke, Nele Gheldof.

Format:

Book

Contributor:

Deplancke, Bart, editor.

Gheldof, Nele, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 786.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 786

Language:

English

Subjects (All):

Medicine.

Human genetics.

Gene expression.

Biomedicine.

Human Genetics.

Gene Expression.

Local Subjects:

Biomedicine.

Human Genetics.

Gene Expression.

Physical Description:

1 online resource (XI, 446 pages) : 108 illustrations, 33 illustrations in color.

Edition:

1.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

Gene regulatory networks play a vital role in organismal development and function by controlling gene expression. With the availability of complete genome sequences, several novel experimental and computational approaches have recently been developed which promise to significantly enhance our ability to comprehensively characterize these regulatory networks by enabling the identification of respectively their genomic or regulatory state components, or the interactions between these two in unprecedented detail. Divided into five convenient sections, Gene Regulatory Networks: Methods and Protocols details how each of these approaches contributes to a more thorough understanding of the composition and function of gene regulatory networks, while providing a comprehensive protocol on how to implement them in the laboratory. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Gene Regulatory Networks: Methods and Protocols aims to provide novices and experienced researchers alike with a comprehensive and timely toolkit to study gene regulatory networks from the point of data generation to processing, visualization, and modeling.

Contents:

How Do You Find Transcription Factors? Computational Approaches to Compile and Annotate Repertoires of Regulators for Any Genome

Expression Pattern Analysis of Regulatory Transcription Factors in Caenorhabditis elegans

High-throughput SELEX Determination of DNA Sequences Bound by Transcription Factors In vitro

Convenient Determination of Protein-Binding DNA Sequences using Quadruple 9-Mer-Based Microarray and DsRed-Monomer Fusion Protein

Analysis of Specific Protein-DNA Interactions by Bacterial One-Hybrid Assay

MITOMI- A Microdluidic Platform for In vitro Characterization of Transcription Factor - DNA Interaction

Detecting Protein-protein Interactions with the Split-Ubiquitin Sensor

Genome-wide Dissection of Post-transcriptional and Post-translational Interactions

Linking Cellular Signalling to Gene Expression using EXT Encoded Reporter Libraries

Sample Preparation for Small RNA Massive Parallel Sequencing

CAGE-Cap Analysis Gene Expression: A Protocol for the Detection of Promoter and Transcriptional Networks

Detecting DNasel-hypersensitivity Sites with MLPA

Detecting Long-range Chromatin Interactions using the Chromosome Conformation Capture Sequencing (3C-seq) Method

Analyzing Transcription Factor Occupancy during Embryo Development using ChIP-seq

Genome-wide Profiling of DNA Binding Proteins using Barcode-based Multiplex Solexa Sequencing

Computational Analysis of Protein-DNA Interactions from ChIP-seq Data

Using a Yeast Inverse One-hybrid System to Identify Functional Binding Sites of Transcription Factors

Using cisTargetX to Predict Transcriptional Targets and Networks in Drosophila

Proteomic Methodologies to Study Transcription Factor Function

A High-throughput Gateway-compatible Yeast One-hybrid Screen to Detect Protein-DNA Interactions

BioTapestry: A Tool to Visualize the Dynamic Properties of Gene Regulatory Networks

Implicit Methods for Qualitative Modeling of Gene Regulatory Networks.

Other Format:

Printed edition:

ISBN:

9781617792922

Access Restriction:

Restricted for use by site license.