Receptor Binding Techniques / edited by Anthony P. Davenport.

Format:

Book

Contributor:

Davenport, Anthony P., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 306.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 306

Language:

English

Subjects (All):

Pharmacy.

Local Subjects:

Pharmacy.

Physical Description:

1 online resource (X, 240 pages) : 71 illustrations.

Edition:

Second Edition.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

Maintaining the high standards set by the successful first edition, Anthony P. Davenport and a panel of hands-on researchers from the pharmaceutical industry and academia take advantage of the latest developments to provide detailed practical methods for studying receptors in silico, in vitro, and in vivo. These readily reproducible techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knock-out mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan polymerase chain reaction (PCR). These ligand binding techniques are perfect for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Receptor Binding Techniques, Second Edition offers academic and commercial researchers in the pharmaceutical and biotechnology industries a set of proven techniques for the successful characterization of receptors and the phenotyping of transgenic animals, including knock-outs.

Contents:

Receptor Databases and Computational Websites for Ligand Binding

Identification of Orphan G Protein-Coupled Receptor Ligands Using FLIPR® Assays

Quantitative Analysis of Orphan G Protein-Coupled Receptor mRNAs by TaqMan® Real-Time PCR

mRNA

Radioligand-Binding and Molecular-Imaging Techniques for the Quantitative Analysis of Established and Emerging Orphan Receptor Systems

Measurement of Radioligand Binding by Scintillation Proximity Assay

Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels

Immunocytochemical Localization of Receptors Using Light and Confocal Microscopy With Application to the Phenotypic Characterization of Knock-Out Mice

Live Cell Imaging of G Protein-Coupled Receptors

Imaging and Characterization of Radioligands for Positron Emission Tomography Using Quantitative Phosphor Imaging Autoradiography

Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography.

Other Format:

Printed edition:

ISBN:

9781592599271

Access Restriction:

Restricted for use by site license.