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Monoclonal Antibody Protocols / edited by William C. Davis.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 45.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 45
- Language:
- English
- Subjects (All):
- Medicine.
- Immunology.
- Biomedicine.
- Local Subjects:
- Biomedicine.
- Immunology.
- Physical Description:
- 1 online resource (X, 264 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 1995.
- System Details:
- text file PDF
- Summary:
- Since the initial description of techniques to immortalize anti- body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain- ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol- ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono- clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga- tors, graduate and postgraduate fellows, and technical staff.
- Contents:
- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies
- Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro
- Culture Conditions that Optimize Outgrowth of Hybridomas
- Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies
- Production of Stable Bovine-Murine Interspecies Hybrids
- Production of Monoclonal Antibodies in Swine
- Production of Monoclonal Antibodies in Horses
- Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies
- Rapid Isotyping of Mouse Monoclonal Antibodies
- Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media
- Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay
- Use of Monoclonal Antibodies in Immunohistochemistry
- Use of Monoclonal Antibodies in Immunoelectron Microscopy
- Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection
- The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry
- Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules
- In Vivo and In Vitro Production of Monoclonal Antibodies
- Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose
- Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies
- Preparation and Use of Immunoaffinity Columns with Monoclonal Antibodies Without Purification from Ascites and Tissue-Culture Medium
- Antigen Purification by Monoclonal Antibody Immunoaffinity Chromatography
- Coupling of Monoclonal Antibodies with Fluorophores
- Coupling of Monoclonal Antibodies with Biotin
- Coupling of Monoclonal Antibodies with Enzymes
- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Negative Selection
- Use of Monoclonal Antibodies with Magnetic Particles to Separate Cell Subpopulations by Positive Selection.
- Other Format:
- Printed edition:
- ISBN:
- 9781592595327
- Access Restriction:
- Restricted for use by site license.
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