DNA Repair Protocols : Eukaryotic Systems / edited by Daryl S. Henderson.

Format:

Book

Contributor:

Henderson, Daryl S., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 113.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 113

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Cytology.

Life Sciences.

Biochemistry, general.

Cell Biology.

Local Subjects:

Life Sciences.

Biochemistry, general.

Cell Biology.

Physical Description:

1 online resource (XXII, 642 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1999.

System Details:

text file PDF

Summary:

In DNA Repair Protocols: Eukaryotic Systems, Daryl S. Henderson and a team of hands-on experts give time-tested instructions for analyzing a wide range of DNA repair processes and cellular responses to DNA damage, including nucleotide and base excision repair, DNA strand break repair, and mismatch repair. The methods focus on eukaryotic model systems that have made, or have the potential to make, important contributions to our understanding of cellular responses to DNA damage in relation to mutagenesis, carcinogenesis, and the cell cycle. Although mammalian cells predominate, consideration is also given to such important nonmammalian model systems as yeast, C. elegans (nematode), Drosophila (fruitfly), Xenopus (amphibian), and plants. DNA Repair Protocols: Eukaryotic Systems offers the most comprehensive collection of DNA repair protocols available, all step-by-step, optimized, and eminently reproducible with tips on troubleshooting and avoiding pitfalls. It will serve as the gold-standard reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches.

Contents:

Mutant Isolation and Gene Cloning

Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe

Isolating Mutants of the Nematode Caenorhabditis elegans That Are Hypersensitive to DNA-Damaging Agents

Isolating DNA Repair Mutants of Drosophila melanogaster

Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis

Screening for ?-Ray Hypersensitive Mutants of Arabidopsis

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating

Strategies for Cloning Mammalian DNA Repair Genes

Novel Complementation Assays for DNA Repair-Deficient Cells

Recognition and Removal of Inappropriate or Damaged DNA Bases

The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair

Mismatch Repair Assay

Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases

A Dot Blot Immunoassay for UV Photoproducts

Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies

Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay

Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis

DNA Damage Quantitation by Alkaline Gel Electrophoresis

The Comet Assay (Single-Cell Gel Test)

Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR

PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair I

PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair II

Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage

Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA

Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts

In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts

Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts

In Vitro Excision Repair Assay in Schizosaccharomyces pombe

Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines

Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes

Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA

Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site

DNA Strand Breakage and Repair

In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts

Physical Monitoring of HO-Induced Homologous Recombination

Use of P Element Transposons to Study DNA Double-Strand Break Repair in Drosophila melanogaster

Analyzing Double-Strand Repair Events in Drosophila

Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks

Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana

Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease

Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells

In Vitro Rejoining of Double-Strand Breaks in Genomic DNA

Extrachromosomal Assay for DNA Double-Strand Break Repair

Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants

DNA Damage Tolerance Mechanisms and Regulatory Responses

Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry

Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos

Inhibition of DNA Synthesis by Ionizing Radiation

Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication

Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts

Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents

Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo

Activation of p53 Protein Function in Response to Cellular Irradiation

Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic Cells by Flow Cytometry

Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry

Immunoassay for Single-Stranded DNA in Apoptotic Cells.

Other Format:

Printed edition:

ISBN:

9781592596751

Access Restriction:

Restricted for use by site license.