Neuronal Cell Death : Methods and Protocols / edited by Laura Lossi, Adalberto Merighi.

Format:

Book

Contributor:

Lossi, Laura, editor.

Merighi, Adalberto, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1254.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1254

Language:

English

Subjects (All):

Neurosciences.

Cytology.

Apoptosis.

Local Subjects:

Neurosciences.

Apoptosis.

Physical Description:

1 online resource (XIV, 396 pages) : 82 illustrations, 48 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neuronal Cell Death: Methods and Protocols seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology.

Contents:

Neuronal Cell Death: An Overview of its Different Forms in Central and Peripheral Neurons

Cell Volume Regulation Monitored with Combined Epifluorescence and Digital Holographic Microscopy

Flow Cytometric Analysis of DNA Synthesis and Apoptosis in Central Nervous System Using Fresh Cell Nuclei

Nuclear Signs of Pre-Neurodegeneration

Multi-Parametric O2 Imaging in Three-Dimensional Neural Cell Models with the Phosphorescent Probes

Calcium Imaging in Neuron Cell Death

Monitoring Mitochondrial Membranes Permeability in Live Neurons and Mitochondrial Swelling through Electron Microscopy Analysis

Real-Time Visualization of Caspase-3 Activation by Fluorescence Resonance Energy Transfer (FRET)

Design and Cloning of Short Hairpin RNAs (shRNAs) into a Lentiviral Silencing Vector to

Genomic Analysis Using Affymetrix Standard Microarray GeneChips (169 Format) in Degenerate Murine Retina

Genomic Analysis of Transcriptional Changes Underlying Neuronal Apoptosis

High-Throughput Cell Death Assays

Image Analysis Algorithms for Immunohistochemical Assessment of Cell Death

In Vitro Oxygen-Glucose Deprivation to Study Ischemic Cell Death

Laser Microbeam Targeting of Single Nerve Axons in Cell Culture

Real-Time Imaging of Retinal Cell Apoptosis by Confocal Scanning Laser Ophthalmoscopy

Targeted Toxicants to Dopaminergic Neuronal Cell Death

Stem Cells, Neural Progenitors, and Engineered Stem Cells

Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant Vectors for Gene Transfer and Gene Therapy

Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors for Gene Transfer and Gene Therapy

Bone Marrow Transplantation for Research and Regenerative Therapies in the Central Nervous System

Detection of Activated Caspase-8 in Injured Spinal Axons by Using Fluorochrome-Labeled Inhibitors of Caspases (FLICA)

Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing

In Vivo Assessment of Neuronal Cell Death in Drosophila

Drosophila Model for Studying Phagocytosis Following Neuronal Cell Death.

Other Format:

Printed edition:

ISBN:

9781493921522

Access Restriction:

Restricted for use by site license.