Developmental Hematopoiesis : Methods and Protocols / edited by Margaret H. Baron.

Format:

Book

Contributor:

Baron, Margaret H., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 105.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine, 1543-1894 ; 105

Language:

English

Subjects (All):

Medicine.

Hematology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Hematology.

Physical Description:

1 online resource (480 pages) : 96 illustrations, 4 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2005.

System Details:

text file PDF

Summary:

Over the past decade technical and conceptual breakthroughs, cross-species comparisons, and multidisciplinary approaches have led to a virtual revolution in developmental biology. In Developmental Hematopoiesis: Methods and Protocols, a panel of expert investigators critically surveys an array of methods available to study hematopoiesis and describes in detail the powerful techniques they have established to investigate embryonic, fetal, and (to a lesser extent) postnatal blood development. The authors apply their approaches to invertebrate (fruit flies) as well as vertebrate (frog, fish, mouse, chick, and human) organisms. These state-of-the-art techniques range from the genetic, molecular, and cellular, to cell and embryo explant culture and whole animals, including in vivo imaging. Bioinformatic and functional genomic approaches for studying stem cells and their supportive stromal cells are also discussed. The protocols follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Developmental Hematopoiesis: Methods and Protocols offers novice and seasoned researchers alike the critical tools needed to gain a fundamental understanding of stem cell biology-examining not only how hematopoietic stem cells form, but also how they differ in the embryo, the fetus, and the adult.

Contents:

Genetic Approaches

Gene Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse

Inducible Transgene Expression in Mouse Stem Cells

Quantitative Trait Analysis in the Investigation of Function and Aging of Hematopoietic Stem Cells

Analysis of Cell Fate Outcomes in Transplantation Models

Flow Cytometric Analysis of Hematopoietic Development

Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells

Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice

Animal Models

Hemocyte Development During Drosophila Embryogenesis

Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos

Fate Mapping Hematopoietic Lineages in the Xenopus Embryo

Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus laevis (J Strain)

Use of Flow Cytometry and Combined DNA Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo

Analysis of Hematopoietic Development in the Zebrafish

Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos

In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo

Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development

Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo

Hematopoietic Stem Cell Development During Mouse Embryogenesis

Analysis of Hematopoietic Progenitors in the Mouse Embryo

In Vivo and In Vitro Assays of Thymic Organogenesis

Retroviral Transduction in Fetal Thymic Organ Culture

Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors

A New Clonal Assay System for Lymphoid and Myeloid Lineages

Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture

Analysis of the Vascular Potential of Hematopoietic Stem Cells

Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture

Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation

Analysis of Hematopoietic Development During Human Embryonic Ontogenesis

Hematopoietic Development of Human Embryonic Stem Cells in Culture

Bioinformatics and Functional Genomics

A Functional Genomics Approach to Hematopoietic Stem Cell Regulation.

Other Format:

Printed edition:

ISBN:

9781592598267

Access Restriction:

Restricted for use by site license.