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Epitope Mapping Protocols : Second Edition / edited by Mike Schutkowski, Ulrich Reineke.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 524.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, Methods and Protocols, 1064-3745 ; 524
- Language:
- English
- Subjects (All):
- Life sciences.
- Immunology.
- Immunoglobulins.
- Biochemistry.
- Life Sciences.
- Biochemistry, general.
- Antibodies.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Immunology.
- Antibodies.
- Physical Description:
- 1 online resource (XIV, 450 pages) : 85 illustrations, 19 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2009.
- System Details:
- text file PDF
- Summary:
- Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.
- Contents:
- B-cell Epitope Mapping
- What Is a B-Cell Epitope?
- Whole Antigen Methods
- Structural Basis of Antibody-Antigen Interactions
- Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy
- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen
- Epitope Mapping by Surface Plasmon Resonance
- Proteolytic Fragmentation for Epitope Mapping
- Epitope Mapping by Proteolysis of Antigen-Antibody Complexes
- Identifying Residues in Antigenic Determinants by Chemical Modification
- Epitope Mapping by Differential Chemical Modification of Antigens
- Peptide Library Approaches
- Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides
- Antibody Epitope Mapping Using SPOT™ Peptide Arrays
- Peptide Microarrays for Profiling of Modification State-Specific Antibodies
- Epitope Mapping Using Phage Display Peptide Libraries
- Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries
- Antibody Specificity Profiling on Functional Protein Microarrays
- Peptide Microarrays for Determination of Cross-Reactivity
- Profiling of Antibody Signatures in Biological Fluids.
- Epitope Mapping Using Randomly Generated Peptide Libraries
- Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease
- Microarrayed Allergen Molecules for Diagnostics of Allergy
- Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays
- Antigen Expressed from Recombinant DNA
- Epitope Mapping Using Homolog-Scanning Mutagenesis
- Epitope Mapping by Region-Specified PCR-Mutagenesis
- Epitope Mapping Using Phage-Display Random Fragment Libraries
- B-Cell Epitope Prediction
- Prediction of Linear B-cell Epitopes
- T-Cell Epitope Mapping
- Molecular Recognition of Diverse Ligands by T-Cell Receptors
- Identification of Human MHC Class I Binding Peptides using the iTOPIA™? Epitope Discovery System
- T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT™? Synthesis
- High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange
- T-Cell Epitope Processing (The Epitope Flanking Regions Matter)
- Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules
- Identification and Validation of T-Cell Epitopes Using the IFN-? EliSpot Assay.
- Other Format:
- Printed edition:
- ISBN:
- 9781597454506
- Access Restriction:
- Restricted for use by site license.
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