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T Cell Protocols : Second Edition / edited by Gennaro De Libero.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 514.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 514
- Language:
- English
- Subjects (All):
- Life sciences.
- Immunology.
- Biochemistry.
- Cell culture.
- Life Sciences.
- Biochemistry, general.
- Cell Culture.
- Local Subjects:
- Life Sciences.
- Biochemistry, general.
- Immunology.
- Cell Culture.
- Physical Description:
- 1 online resource (XII, 166 pages) : 41 illustrations, 3 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2009.
- System Details:
- text file PDF
- Summary:
- With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls. Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.
- Contents:
- Analysis of Frequency and Phenotype of Antigen-Specific T Cells
- B Cell Helper Assays
- transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing
- Flow Cytometry and Cell Activation
- Investigating T Cells by Polychromatic Flow Cytometry
- Generation of Human T Cell Clones
- Limiting Dilution Analysis of Antigen-Specific T Cells
- T Cell Epitope-Mapping by Cytokine Gene Expression Assay
- Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications
- Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE
- Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation.
- Other Format:
- Printed edition:
- ISBN:
- 9781603275279
- Access Restriction:
- Restricted for use by site license.
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