Calcium Signaling Protocols / edited by David G. Lambert.

Format:

Book

Contributor:

Lambert, David G., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 312.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 312

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Physical Description:

1 online resource (X, 359 pages) : 61 illustrations.

Edition:

Second Edition.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition continues the excellence of the highly regarded first edition. New to this edition are chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for novice and expert researchers alike both theoretical and practical information for the optimal design and performance of studies of intracellular Ca2+ regulation.

Contents:

General

Fluorescent Measurement of [Ca2+]c

Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2

Measurement of [Ca2+]i in Cell Suspensions Using Indo-1

Specialist Measurement Systems

Confocal Microscopy

Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration

Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer

Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader

Measurement of Ca2+ Channel Activity

Measurement of Ca2+ Entry Using 45Ca2+

Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding

Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents

Measurement of Ins(1,4,5,)P3 and Ca2+ Release From Intracellular Stores

Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells

Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay

Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay

Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells

Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators

Specialist Measurement Techniques

Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry

Measurement of Calcium and Movement in Heart Cells

Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations

Measurement of Cytosolic-Free Ca2+ in Plant Tissue

Ca2+-Sensitive Targets

Assay and Purification of Calmodulin-Dependent Protein Kinase

Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation

Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1).

Other Format:

Printed edition:

ISBN:

9781592599493

Access Restriction:

Restricted for use by site license.