Protein NMR Techniques / edited by Alexander Shekhtman, David S. Burz.

Format:

Book

Contributor:

Shekhtman, Alexander, editor.

Burz, David S., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 831.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 831

Language:

English

Subjects (All):

Life sciences.

Spectrum analysis.

Proteins.

Life Sciences.

Protein Science.

Spectroscopy/Spectrometry.

Local Subjects:

Life Sciences.

Protein Science.

Spectroscopy/Spectrometry.

Physical Description:

1 online resource (XIV, 518 pages) : 107 illustrations, 23 illustrations in color.

Edition:

Third edition 2012.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein NMR Techniques, Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.

Contents:

A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins

Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

Isotope Labeling in Insect Cells

Isotope Labeling in Mammalian Cells

Cell-free Protein Production for NMR Studies

Cell-free Membrane Protein Expression for Solid-state NMR

Expression and Purification of Src-family Kinases for Solution NMR Studies

NMR Studies of Large Protein Systems

Protein Dynamics by 15N Nuclear Magnetic Relaxation

Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

NMR Studies of Protein-RNA Interactions

Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies

NMR Studies of Protein-ligand Interactions

In-cell NMR Spectroscopy in Escherichia coli

Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR

Solid-State NMR Spectroscopy of Protein Complexes

Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2

Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility

Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy

Comprehensive Automation for NMR Structure Determination of Proteins

ARIA for Solution and Solid-state NMR Protein NMR Techniques

Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS. .

Other Format:

Printed edition:

ISBN:

9781617794803

Access Restriction:

Restricted for use by site license.