Suicide Gene Therapy : Methods and Reviews / edited by Caroline J. Springer.

Format:

Book

Contributor:

Springer, Caroline J., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 90.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 90

Language:

English

Subjects (All):

Medicine.

Oncology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Oncology.

Physical Description:

1 online resource (XIV, 555 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2004.

System Details:

text file PDF

Summary:

Suicide gene therapy has become a major element in anticancer gene therapy and holds great promise for the future. In Suicide Gene Therapy: Methods and Reviews, prominent researchers and clinicians comprehensively detail the theory and practice of this exciting and elegant therapeutic approach. In their multidisciplinary treatment, the authors cover all the major aspects of suicide gene therapy, including the design and use of vectors in gene transduction, various enzyme and prodrug systems, the mechanistic analysis of the bystander effect, the design and synthesis of prodrugs, the immunological implications, and its clinical impact. They also describe and expound upon all the cutting-edge methods needed to explore, study, and advance understanding of the basic biology underlying gene therapy. Each fully tested method includes step-by-step instructions, a discussion of the principle behind the technique, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and notes on the interpretation and use of results. Authoritative and up-to-date, Suicide Gene Therapy: Methods and Reviews not only summarizes our knowledge of suicide gene therapy today, but also provides the experimental means to advance its future development and therapeutic applications.

Contents:

to the Background, Principles, and State of the Art in Suicide Gene Therapy

to Vectors for Suicide Gene Therapy

Construction of VNP20009

Nonreplicating DNA Viral Vectors for Suicide Gene Therapy

Replication-Selective Oncolytic Adenoviruses

Retroviral Vectors for Suicide Gene Therapy

Nonviral Liposomes

Peptide- and Polymer-Based Gene Delivery Vehicles

Design of Prodrugs for Suicide Gene Therapy

Cytochrome P450-Based Gene Therapies for Cancer

Tumor Sensitization to Purine Analogs by E. coli PNP

Enzyme-Prodrug Systems

Methods to Improve Efficacy in Suicide Gene Therapy Approaches

Extracellular ?-Glucuronidase for Gene-Directed Enzyme-Prodrug Therapy

Enhancement of Suicide Gene Prodrug Activation by Random Mutagenesis

Combination Suicide Gene Therapy

Immune Response to Suicide Gene Therapy

Targeting Cancer With Gene Therapy Using Hypoxia as a Stimulus

Radiation-Activated Antitumor Vectors

In Vitro and In Vivo Models for Evaluation of GDEPT

Suicide Gene Therapy in Liver Tumors

Clinical Trials With GDEPT

The Nitroreductase/CB1954 Enzyme-Prodrug System

Side Effects of Suicide Gene Therapy

Antibody-Directed Enzyme-Prodrug Therapy

Bioreductive Prodrugs for Cancer Therapy.

Other Format:

Printed edition:

ISBN:

9781592594290

Access Restriction:

Restricted for use by site license.