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Cancer Cell Signaling : Methods and Protocols / edited by David M. Terrian.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 218.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 218
- Language:
- English
- Subjects (All):
- Medicine.
- Oncology.
- Medicine & Public Health.
- Local Subjects:
- Medicine & Public Health.
- Oncology.
- Physical Description:
- 1 online resource (XV, 333 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2003.
- System Details:
- text file PDF
- Summary:
- The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid. Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.
- Contents:
- Manipulation and Detection of Survival Signals
- Functional Analysis of the Antimitogenic Activity of Tumor Suppressors
- Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination
- Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells
- The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress
- Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
- Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
- TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)
- Manipulation and Edtection of Oncogenic Signals
- Kinetworks™ Protein Kinase Multiblot Analysis
- Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers
- Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay
- Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/In Situ Assay
- Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells
- Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD)
- Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model
- Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer
- Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes
- Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes
- Protein Interactions
- Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology
- Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
- Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
- Genomic Rearrangements
- Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing
- Clonality Analysis by T-Cell Receptor ? PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL).
- Other Format:
- Printed edition:
- ISBN:
- 9781592593569
- Access Restriction:
- Restricted for use by site license.
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