Expression Profiling in Neuroscience / edited by Yannis Karamanos.

Format:

Book

Contributor:

Karamanos, Yannis, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 64.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 64

Language:

English

Subjects (All):

Neurosciences.

Proteomics.

Local Subjects:

Neurosciences.

Proteomics.

Physical Description:

1 online resource (XIII, 284 pages) : 55 illustrations, 33 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

Transcriptomics and proteomics, studying the profile of the expression of nucleic acids and proteins respectively, are increasingly applied to gain a mechanistic insight into a wide spectrum of investigation, and the use of expression profiling studies for the central nervous system and brain function aids in the understanding of neurodegenerative disorders and tumor development mechanisms. In Expression Profiling in Neuroscience, expert researchers provide a survey of the most commonly used approaches in the field and scan the different ways of studying the central nervous system/brain environment through expression profiling. The first part addresses the gene expression profiling of the brain at a large scale or to a specific cell type such as blood-brain barrier endothelium. Then, the second part describes the protein expression studies and the different technologies applied. Written for the popular Neuromethods series, chapters include corresponding background information, tested laboratory protocols, and step-by-step methods for reproducible laboratory experiments. Detailed and authoritative, Expression Profiling in Neuroscience presents the state-of-the-art techniques necessary to expand research further into this vital area of study.

Contents:

DNA Microarrays for Gene Expression Analysis in Brain Tissue and Cell Lines

Gene Expression Profiling Using the Terminal Continuation (TC) RNA Amplification Method for Small Input Samples in Neuroscience

Expression Profiling In Brain Disorders

Endothelial Cell Heterogeneity of Blood-Brain Barrier Gene Expression: Analysis by LCM/qRT-PCR

Gene Expression Profiling Using 3' Digital Tag Approach

Sharing Expression Profiling Data with Gemma

Two-Dimensional Protein Analysis of Neural Stem Cells

iTRAQ Proteomics Profiling of Regulatory Proteins During Oligodendrocyte Differentiation

Protein Profiling of the Brain: Proteomics of Isolated Tissues and Cells

The Proteome of Brain Capillary Endothelial Cells: Towards a Molecular Characterization of an In Vitro Blood-Brain Barrier Model

MALDI Imaging Technology Application in Neurosciences: From History to Perspectives

Profiling of HIV Proteins in Cerebrospinal Fluid

Proteomic Profiling of Cerebrospinal Fluid

New Nanotechnology Applications in Single Cell Analysis: Why and How.

Other Format:

Printed edition:

ISBN:

9781617794483

Access Restriction:

Restricted for use by site license.