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Cell-Based Microarrays : Methods and Protocols / edited by Ella Palmer.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 706.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 706
- Language:
- English
- Subjects (All):
- Life sciences.
- Cytology.
- Life Sciences.
- Cell Biology.
- Local Subjects:
- Life Sciences.
- Cell Biology.
- Physical Description:
- 1 online resource (X, 209 pages) : 46 illustrations, 10 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2011.
- System Details:
- text file PDF
- Summary:
- As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
- Contents:
- Cell-Based Microarrays: Overview
- Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate
- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays
- A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays
- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays
- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells
- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays
- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA)
- Reverse Transfected Cell Microarrays in Infectious Disease Research
- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies
- Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging
- Immobilized Culture and Transfection Microarray of Nonadherent Cells
- Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays
- Polymer Microarrays for Cellular High-Content Screening
- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation
- Microfluidic Image Cytometry.
- Other Format:
- Printed edition:
- ISBN:
- 9781617379703
- Access Restriction:
- Restricted for use by site license.
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