2 options
Plant Metabolic Flux Analysis : Methods and Protocols / edited by Martine Dieuaide-Noubhani, Ana Paula Alonso.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1090.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1090
- Language:
- English
- Subjects (All):
- Life sciences.
- Botanical chemistry.
- Metabolism.
- Botany.
- Life Sciences.
- Plant Sciences.
- Plant Biochemistry.
- Metabolomics.
- Local Subjects:
- Life Sciences.
- Plant Sciences.
- Plant Biochemistry.
- Metabolomics.
- Physical Description:
- 1 online resource (XI, 365 pages) : 75 illustrations, 31 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press : Imprint: Humana Press, 2014.
- System Details:
- text file PDF
- Summary:
- In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Plant Metabolic Flux Analysis, seeks to aid scientists in the further study of cutting-edge protocols and methodologies that are crucial to getting ahead in MFA.
- Contents:
- Application of Metabolic Flux Analysis to Plants
- Metabolic Network Reconstruction and Their Topological Analysis
- 14C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism
- Optimization of Steady-state 13C-labeling Experiments for Metabolic Flux Analysis
- Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy
- Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR
- Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS
- Quantifying 13C-labeling in Free Sugars and Starch by GC-MS
- Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway
- In Vivo NMR for 13C metabolic Flux Analysis
- Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification
- Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism
- Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis
- High-throughput Data Pipelines for Metabolic Flux Analysis in Plants
- Analysis of Enzyme Activities
- Analytical Kinetic Modeling: A Practical Procedure
- Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling
- Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling
- Plant Genome-Scale Modeling and Implementation
- 34S and 15N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency
- Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants.
- Other Format:
- Printed edition:
- ISBN:
- 9781627036887
- Access Restriction:
- Restricted for use by site license.
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.