Controlled Genetic Manipulations / edited by Alexei Morozov.

Format:

Book

Contributor:

Morozov, Alexei, editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 65.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 65

Language:

English

Subjects (All):

Neurosciences.

Human genetics.

Human Genetics.

Local Subjects:

Neurosciences.

Human Genetics.

Physical Description:

1 online resource (XII, 227 pages) : 48 illustrations, 27 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2012.

System Details:

text file PDF

Summary:

The current demand for the development of techniques for controlled genetic manipulations is driven by the anatomical and physiological complexity of the brain and by the need for experimental models that can address this complexity through selective manipulation of defined components of the system: specific neuronal populations or selected synapses. In Controlled Genetic Manipulations, expert researchers present detailed chapters to supply basic technical information about controlled genetic manipulations and to provide examples of creative implementation of this methodology when addressing a unique biological problem. Some chapters of the book describe the most recent developments in the basic methodology, which includes use of Cre-recombinase, methods for delivery of genetic material into the brain and the use of optogenetics, whereas other chapters focus on applying these techniques to addressing particular biological questions like structural and functional mapping of neuronal circuits, analysis of specific synaptic connections, modeling or gene therapy of neurological disorders. As part of the Neuromethods series, chapters include key implementation advice that is crucial for getting optimal results. Authoritative and practical, Controlled Genetic Manipulations serves as a valuable guide to a variety of techniques, provided by many of the pioneers of the approaches.

Contents:

Regulation of Cre Recombinase: Use of Ligand-Regulated and Dimerizable Cre for Transgenesis

Genetic Manipulations Using Cre and Mutant loxP Sites

Using Recombinant Adeno-Associated Viral Vectors for Gene Expression in the Brain

Using Lentiviral Vectors as Delivery Vehicles for Gene Therapy

Targeting Neurological Disease with siRNA

In Utero Electroporation for Cellular Transgenesis in the Developing Mammalian Forebrain

Single-Cell Electroporation of siRNA in Primary Neuronal Cultures

Development and Application of Membrane-Tethered Toxins for Genetic Analyses of Neuronal Circuits

Genetic Methods for Anatomical Analysis of Neuronal Circuits

Functional Circuitry Analysis in Rodents Using Neurotoxins/Immunotoxins

Analysis of Neuronal Circuits with Optogenetics.

Other Format:

Printed edition:

ISBN:

9781617795336

Access Restriction:

Restricted for use by site license.