Microsatellites : Methods and Protocols / edited by Stella K. Kantartzi.

Format:

Book

Contributor:

Kantartzi, Stella K., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1006.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1006

Language:

English

Subjects (All):

Medicine.

Human genetics.

Biomedicine.

Human Genetics.

Local Subjects:

Biomedicine.

Human Genetics.

Physical Description:

1 online resource (XI, 339 pages) : 71 illustrations, 43 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.

System Details:

text file PDF

Summary:

Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.

Contents:

Microsatellites: Evolution and Contribution

Screening of Genomic Libraries

PCR-Based Identification of Microsatellite Arrays (PIMA)

Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO)

Microsatellite DNA Capture from Enriched Libraries

Next-Generation Sequencing for High-Throughput Molecular Ecology: A Step-by-Step Protocol for Targeted Multilocus Genotyping by Pyrosequencing

Optimizing Selection of Microsatellite Loci from 454 Pyrosequencing Via Post-Sequencing Bioinformatic Analyses

Identification of DNA-Microsatellite Markers for the Characterization of Somatic Embryos in Quercus suber

Simple Sequence Repeats (SSR) Amplification

Microsatellite Amplification in Plants: Optimization Procedure of Major PCR Components

Development of a Multiplex PCR Assay for Characterization of Embryonic Stem Cells

Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis for Visualization of Single Sequence Repeats

Microsatellite Fragment Analysis Using the ABI Prism® 377 DNA Sequencer

Robust and Inexpensive SSR Markers Analyses Using LI-COR DNA Analyzer

The Use of the MegaBACE for Sequencing and Genotype Analysis

Analyzing Microsatellites Using the QIAxcel System

Microsatellite Analysis of Malaria Parasites

Informativeness of Microsatellite Markers

Microsatellite Data Analysis for Population Genetics

Molecular Mapping and Breeding with Microsatellite Markers.

Other Format:

Printed edition:

ISBN:

9781627033893

Access Restriction:

Restricted for use by site license.