Tumor Suppressor Genes : Volume 2 Regulation, Function, and Medicinal Applications / edited by Wafik S. El-Deiry.

Format:

Book

Contributor:

El-Deiry, Wafik S., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 223.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 223

Language:

English

Subjects (All):

Medicine.

Oncology.

Medicine & Public Health.

Local Subjects:

Medicine & Public Health.

Oncology.

Physical Description:

1 online resource (XIX, 657 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2003.

System Details:

text file PDF

Summary:

Powerful new tools are now available to discover and understand tumor suppressor genes (TSGs) and the biochemical mechanisms by which they control cancer development and progression. In Tumor Suppressor Genes, Volume 2: Regulation, Function, and Medicinal Applications, leading physician scientists and researchers explore the cell biology and biochemical function of the tumor suppressor genes, as well as their physiological role in vivo. The authors detail the physical methods-NMR, microarray approaches, posttranslational structure analysis, analysis of regulation at the gene expression and protein signaling levels-used to understand the function of tumor suppressor genes. In vivo approaches discussed include studies in yeast, Drosophila, mice, and human tumors. The authors also discuss how one ultimately derives a "tumor progression model," the drug discovery process, and the weaknesses of cancers that allow drugs to selectively kill them. Other aspects reviewed include screening strategies, targeting the blood supply of tumors, and the replacement of defective cancer genes to achieve a therapeutic effect. The companion volume of this set, Tumor Suppressor Genes, Volume 1: Pathways and Isolation Strategies, covers all known tumor suppressor genes, describing their cellular signaling pathways, how they were discovered, how they can be analyzed, and how they can be optimally used in cancer therapeutics Comprehensive and authoritative, the two volumes of Tumor Suppressor Genes provide an unparalleled compilation of key data on all known tumor suppressor pathways and a treasury of techniques for their discovery, analysis, and uses in cancer therapeutics.

Contents:

Understanding the Function and Regulation of Tumor Suppressor Genes

Utilizing NMR to Study the Structure of Growth-Inhibitory Proteins

Generation and Application of Phospho-specific Antibodies for p53 and pRB

Stability and Ubiquitination of the Tumor Suppressor Protein p53

Role of Tumor Suppressors in DNA Damage Response

In Situ Hybridization in Cancer and Normal Tissue

Genetic Strategies in Saccharomyces cerevisiae to Study Human Tumor Suppressor Genes

Electrophoretic Mobility Shift Analysis of the DNA Binding of Tumor Suppressor Gene Products

Analysis of Gene Promoter Regulation by Tumor Suppressor Genes

DNA Footprinting

Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation

Co-immunoprecipitation of Tumor Suppressor Protein-Interacting Proteins

Microarray Approaches for Analysis of Tumor Suppressor Gene Function

Analysis of Tumor Suppressor Gene-Induced Senescence

Yeast Two-Hybrid Screening as a Means of Deciphering Tumor Suppressor Pathways

Somatic Cell Knockouts of Tumor Suppressor Genes

Colony Growth Suppression by Tumor Suppressor Genes

Flow Cytometric Analysis of Cell Cycle Control by Tumor Suppressor Genes

Analysis of Cyclin-Dependent Kinase Activity

Tumor Suppressor Gene-Inducible Cell Lines

In Vitro Models of Early Neoplastic Transformation of Human Mammary Epithelial Cells

Tumor Suppression Through Angiogenesis Inhibition

Flow Cytometric Analysis of Tumor Suppressor Gene-Induced Apoptosis

Functional Analysis of Tumor Suppressor Genes in Mice

The Tissue Microenvironment as an Epigenetic Tumor Modifier

Analyzing the Function of Tumor Suppressor Genes Using a Drosophila Model

Assembling a Tumor Progression Model

Conformation-Sensitive Gel Electrophoresis for Detecting BRCA1 Mutations

Medicinal Applications of Tumor Suppessors

Conversion Technology and Cancer Predispositions

Discovering Novel Anticancer Drugs

Targets in Apoptosis Signaling

Tumor Deprivation of Oxygen and Tumor Suppressor Gene Function

Hormonal and Differentiation Agents in Cancer Growth Suppression

Regulation of NF-?B by Oncoproteins and Tumor Suppressor Proteins

Blocking Survivin to Kill Cancer Cells

Targeting the Mitochondria to Enhance Tumor Suppression

Novel Approaches to Screen for Anticancer Drugs Using Saccharomyces cerevisiae

Tumor Suppressor Gene Therapy

Therapeutic Strategies Using Inhibitors of Angiogenesis

Isolation of p53 Inhibitors by Screening Chemical Libraries in Cell-Based Readout System.

Other Format:

Printed edition:

ISBN:

9781592593293

Access Restriction:

Restricted for use by site license.