Herpes Simplex Virus Protocols / edited by S. Moira Brown, Alasdair R. MacLean.

Format:

Book

Contributor:

Brown, S. Moira, editor.

MacLean, Alasdair R., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 10.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine, 1543-1894 ; 10

Language:

English

Subjects (All):

Life sciences.

Microbiology.

Life Sciences.

Local Subjects:

Life Sciences.

Microbiology.

Physical Description:

1 online resource (XI, 419 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

Herpes Simplex Virus Protocols comprises a wide range of experimental protocols that should be especially useful to new workers in herpes virology. Hopefully, it will also provide information for those with experience in the field, as well as those embarking on techniques that are new to them. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide protocols dealing with those procedures that are most widely used; and we have selected expert authors accordingly. We have also tried to cover the range from the more biological in vivo maneuvers to purely molecular procedures, taking into account the topical interest in the potential use of HSV as a therapeutic tool. In this way there should be sufficient inf- mation for most procedures the average herpes virologist is likely to require-- at least at this moment in time! Since the herpesviruses are a large family, we have largely based the protocols on the virus we know best--herpes simplex virus. With this as the prototype, it should be relatively easy to extrapolate and make the necessary modifications required for application to some of the other herpesviruses, especially members of the alpha group, such as PRV and EHV. It would have been an impossible task to include chapters covering the unique aspects of each known herpesvirus.

Contents:

HSV Growth, Preparation, and Assay

HSV Entry and Spread

Preparation of HSV-DNA and Production of Infectious Virus

HSV Mutagenesis

Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions

Construction and Use of Cell Lines Expressing HSV Genes

Analysis of HSV Polypeptides

Protein Purification

Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells

Crystallization of Macromolecules for Three-Dimensional Structure Determination

Direct Immunogold Labeling of Herpesvirus Suspensions

Expression of HSV Proteins in Bacteria

In Vitro Systems to Analyze HSV Transcript Processing

Analysis of HSV-1 Transcripts by RNA-PCR

Transient Assays for HSV Origin and Replication Protein Function

HSV Amplicons in Gene Therapy

Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions

HSV-Cellular Protein Interactions

Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse

Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit

HSV Latency In Vitro

Assessing Cell-Mediated Immune Responses to HSV in Murine Systems

Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection

Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction

HSV Vectors for Gene Therapy

Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.

Other Format:

Printed edition:

ISBN:

9781592595945

Access Restriction:

Restricted for use by site license.