RNA Interference : Challenges and Therapeutic Opportunities / edited by Mouldy Sioud.

Format:

Book

Contributor:

Sioud, Mouldy, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1218.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1218

Language:

English

Subjects (All):

Medicine.

Human genetics.

Pharmacology.

Biomedicine.

Pharmacology/Toxicology.

Human Genetics.

Local Subjects:

Biomedicine.

Pharmacology/Toxicology.

Human Genetics.

Physical Description:

1 online resource (XVI, 495 pages) : 108 illustrations, 58 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting, and methods to minimize siRNA's unwanted effects. Preclinical and clinical use of synthetic siRNAs, the roles of miRNAs in cancer, and the promise of extracellular miRNAs for diagnosis are also covered in this meticulous collection, along with novel methods for identifying endogenous siRNAs and the annotation of small RNA transcriptomes. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Comprehensive and cutting-edge, RNA Interference: Challenges and Therapeutic Opportunities will aid researchers, clinicians, teachers, and biotechnologists interested in the power of RNA-based therapies.

Contents:

RNA Interference: Mechanisms, Technical Challenges and Therapeutic Opportunities

Nanostructured RNAs for RNA Interference

One Long Oligonucleotide or Two Short Oligonucleotides Based shRNA Construction and Expression

PLGA Microspheres Encapsulating siRNA

Magnetic Nanoparticle and Magnetic Field Assisted siRNA Delivery In Vitro

Cytoplasmic Delivery of siRNAs to Monocytes and Dendritic Cells Via Electroporation

Delivery of siRNAs to Cancer Cells Via Bacteria

Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy

Modulating the Tumor Microenvironment with RNA Interference as a Cancer Treatment Strategy

Targeted In Vivo Delivery of siRNA and an Endosome-Releasing Agent to Hepatocytes

Cell-Internalization SELEX: Method for Identifying Cell-Internalizing RNA Aptamers for Delivering siRNAs to Target Cells

Strategies for siRNA Navigation to Desired Cells

Analysis of RNA Interference-Mediated Hepatic Gene Silencing Following Murine Hydrodynamic Injection of Guanidinopropyl-Modified siRNAs

Therapy of Respiratory Viral Infections with Intranasal siRNAs

Engineering Therapeutic Cancer Vaccines that Activate Anti-Tumor Immunity

Immunosuppressive Factor Blockade in Dendritic Cells Via siRNAs Results in Objective Clinical Responses

Targeting bcr-abl Transcripts with siRNAs in an Imatinib-Resistant Chronic Myeloid Leukemia Patient: Challenges and Future Directions

A Facile Method for Interfering with Off-Target Silencing Mediated by the Sense Strand

Overcoming the Challenges of siRNA Activation of Innate Immunity: Design Better Therapeutic siRNAs

Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

In Silico Identification of Novel Endo-siRNAs

Computer-Assisted Annotation of Small RNA Transcriptomes

miR-CATCH: MicroRNA Capture Affinity Technology

Roles of MicroRNAs in Cancers and Development

Targeting MicroRNAs to Withstand Cancer Metastasis

Urinary MicroRNAs as a New Class of Non-Invasive Biomarkers in Oncology, Nephrology and Cardiology

Magnetic Bead-Based Isolation of Exosomes

Modified 2'-Ribose Small RNAs Function as Toll-Like Receptor-7/8 Antagonists.

Other Format:

Printed edition:

ISBN:

9781493915385

Access Restriction:

Restricted for use by site license.