Receptor Signal Transduction Protocols / edited by R. A. J. Challiss.

Format:

Book

Contributor:

Challiss, R. A. J. (R. A. John), editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 83.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 83

Language:

English

Subjects (All):

Medicine.

Neurosciences.

Biomedicine.

Local Subjects:

Biomedicine.

Neurosciences.

Physical Description:

1 online resource (XIII, 275 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1997.

System Details:

text file PDF

Summary:

R. A. J. Challiss assembles in Receptor Signal Transduction Protocols an outstanding collection of state-of-the-art methods from leading experts for interrogating ligand-receptor and receptor-effector interactions. These powerful methods emphasize the regulation of G-protein-coupled receptors and focus on receptor characterization, methods for investigating how receptors couple to G-proteins, and approaches to studying how receptor expression and function is regulated. Designed to be easily reproducible, the protocols offered include chimeric receptor construction, antibody generation, transient and stable transfection methods, in situ hybridization, as well as immunocytochemistry, functional assays of G-protein function, antisense methods for defining receptor-effector coupling pathways, and many more. Receptor Signal Transduction Protocols blends authoritative descriptions and applications of well-established methodologies with many novel techniques at the cutting edge of signal transduction research. The coverage cuts across disciplines and allows those already well-versed in one area to expand their repertoire of potent methods and broaden their research horizons. Every researcher interested in signal transduction today will find this richly practical guide indispensable.

Contents:

Methods for Characterizing Receptors

Radioligand-Binding Methods for Membrane Preparations and Intact Cells

Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding

Approaches to the Stable Transfection of G Protein-Coupled Receptors

Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels

The Generation of Receptor-Selective Antibodies

In Situ Hybridization

lmmunocytochemical Methods for Investigating Receptor Localization

Methods for Studying Receptor Coupling Mechanisms

Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes

Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTP?S Binding

Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes

Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling

Identification and Quantitation of G Protein ?-Subunits

Covalent Modification of G Proteins by Affinity Labeling

Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection

Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C

Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons

Methods for Investigating the Regulation of Receptor Function

Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation

Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation

Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells

Regulation of Receptor Expression.

Other Format:

Printed edition:

ISBN:

9781592595679

Access Restriction:

Restricted for use by site license.