Antifungal Agents : Methods and Protocols / edited by Erika J. Ernst, P. David Rogers.

Format:

Book

Contributor:

Ernst, Erika J., editor.

Rogers, P. David, editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 118.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 118

Language:

English

Subjects (All):

Medicine.

Infectious diseases.

Medicine & Public Health.

Infectious Diseases.

Local Subjects:

Medicine & Public Health.

Infectious Diseases.

Physical Description:

1 online resource (X, 210 pages) : 30 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

With the growing frequency of fungal infections even as resistance to existing antifungal agents increases, methods for finding novel drug targets and evaluating antifungal resistance have become more important than ever. In Antifungal Agents: Methods and Protocols, expert scientists describe in detail the state-of-the-art molecular methods they have optimized for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. Many of the protocols were developed in the laboratory and, when a method is limited to specific organisms, include alternative techniques to expand their application. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists, and molecular biologists the productive tools they need today in order to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.

Contents:

Susceptibility Testing Methods of Antifungal Agents

Antifungal Resistance

Deoxyribonucleic Acid Fingerprinting Methods for Candida Species

CARE-2 Fingerprinting of Candida albicans Isolates

Targeted Gene Deletion in Candida albicans Wild-Type Strains by MPA R Flipping

Application of Deoxyribonucleic Acid Microarray Analysis to the Study of Azole Antifungal Resistance in Candida albicans

Application of Proteomic Analysis to the Study of Azole Antifungal Resistance in Candida albicans

Techniques for Antifungal Susceptibility Testing of Candida albicans Biofilms

Antifungal Development and Evaluation

Natural Products and Antifungal Drug Discovery

Use of an Animal Model of Disseminated Candidiasis in the Evaluation of Antifungal Therapy

Murine Model of Invasive Aspergillosis

Antifungal Combinations

Postantifungal Effect Methods

Host Responses and Immunotherapy

Evaluation of Immunotherapy in Invasive Candidiasis

Interaction of Histoplasma capsulatum With Human Macrophages, Dendritic Cells, and Neutrophils

Immunology of Infection Caused by Cryptococcus neoformans.

Other Format:

Printed edition:

ISBN:

9781592599431

Access Restriction:

Restricted for use by site license.