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The Urinary Proteome : Methods and Protocols / edited by Alex J. Rai.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Rai, Alex J. (Alex Jaideep), editor.
SpringerLink (Online service)
Series:
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 641.
Springer Protocols (Springer-12345)
Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 641
Language:
English
Subjects (All):
Life sciences.
Biochemistry.
Proteomics.
Proteins.
Life Sciences.
Biochemistry, general.
Protein Science.
Local Subjects:
Life Sciences.
Biochemistry, general.
Protein Science.
Proteomics.
Physical Description:
1 online resource (VI, 358 pages) : 82 illustrations, 4 illustrations in color.
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press, 2010.
System Details:
text file PDF
Summary:
Urine is one of the most easily accessible biological samples, and it provides a treasure trove of molecules essential for clinical diagnostics. In The Urinary Proteome: Methods and Protocols, expert researchers review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state of the art methods for proteomic analysis using urine specimens. Featuring the most recent advances in sample preparation, data analysis, and methods and applications, the book contains a multitude of detailed methods, including procedural details for the identification and characterization of urine biomarkers, which hold great potential for the diagnosis and treatment of many different disease conditions. Written in the highly successful Methods in Molecular Biology™ series format, chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and systematic, The Urinary Proteome: Methods and Protocols is an ideal guide for scientific researchers, clinical laboratorians, clinical and translational scientists, and others interested in the vital study of proteomics and biomarker discovery.
Contents:
to Urinalysis: Historical Perspectives and Clinical Application
A Primer on Clinical Applications and Assays Using Urine: Focus on Analysis of Plasma Cell Dyscrasias Using Automated Electrophoresis and Immunofixation
Application of Free Flow Electrophoresis to the Analysis of the Urine Proteome
Standardized Preprocessing of Urine for Proteome Analysis
Different Sample Preparation and Detection Methods for Normal and Lung Cancer Urinary Proteome Analysis
Isolation and Purification of Exosomes in Urine
Bioinformatics of the Urinary Proteome
PROTIS: Use of Combined Biomarkers for Providing Diagnostic Information on Disease States
Statistical Contributions to Proteomic Research
A Review of Experimental Design Best Practices for Proteomics Based Biomarker Discovery: Focus on SELDI-TOF
Urine Proteomic Profiling for Biomarkers of Acute Renal Transplant Rejection
Surface Plasmon Resonance Biosensorics in Urine Proteomics
Urinary Proteins for the Diagnosis of Obstructive Sleep Apnea Syndrome
Immune Response Biomarker Profiling Application on ProtoArray® Protein Microarrays
Design and Validation of an Immunoaffinity LC-MS/MS Assay for the Quantification of a Collagen Type II Neoepitope Peptide in Human Urine: Application as a Biomarker of Osteoarthritis
Cell-Specific Biomarkers in Renal Medicine and Research
Proteomic Assays for the Detection of Urothelial Cancer
Urine Proteomic Analysis: Use of Two-Dimensional Gel Electrophoresis, Isotope Coded Affinity Tags, and Capillary Electrophoresis
Proteomic Analysis of Pancreatic Secretory Trypsin Inhibitor/Tumor-Associated Trypsin Inhibitor from Urine of Patients with Pancreatitis or Prostate Cancer.
Other Format:
Printed edition:
ISBN:
9781607617112
Access Restriction:
Restricted for use by site license.

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