Antiviral Methods and Protocols / edited by Derek Kinchington, Raymond F. Schinazi.

Format:

Book

Contributor:

Kinchington, Derek, editor.

Schinazi, Raymond F., editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 24.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 24

Language:

English

Subjects (All):

Medicine.

Infectious diseases.

Medicine & Public Health.

Infectious Diseases.

Local Subjects:

Medicine & Public Health.

Infectious Diseases.

Physical Description:

1 online resource (XIV, 404 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2000.

System Details:

text file PDF

Summary:

In Antiviral Methods and Protocols, experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. The detailed protocols also discuss possible pitfalls and ways to overcome them. Timely and comprehensive, Antiviral Methods and Protocols offers today's researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections.

Contents:

Changing Methods for Discovering Antiviral Drugs

Changing Methods for Discovering Antiviral Drugs

Laboratory Safety Considerations

Routine Quality Control Testing of Cell Cultures

Hepatitis Virus

Hep AD38 Assay

Hepatitis B Virus Cell Culture Assays for Antiviral Activity

A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase

Assays for Measuring Hepadnaviral Supercoiled DNA

Assays for Hepatitis B Virus DNA-and RNA-Dependent DNA Polymerase Activities

Development of a Hepatitis C Virus RNA Helicase High Throughput Assay

Herpesvirus

Determination of Viral Infectivity

Antiviral Screening Assays for Human Herpesviruses 6 and 7

Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture

Monitoring Drug Resistance for Herpesviruses

Herpesvirus Protease Assays

Human Immunodeficiency Virus

Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV

Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells

Assessment of Activity of Topical Virucidal Agents

Assays That Measure Selective Killing of Virus-Infected Cells

Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs

Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase

RT-PCR for Amplification of Specific Fragments of HIV-1 Genome

Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase

Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay

Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses

Identifying and Characterizing HIV Protease Inhibitors

Retroviral Integrase

Human Papilloma Virus

Papillomavirus DNA Replication

Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells

Influenza Virus

Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en

Virological Methods for the Generation and Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en.

Other Format:

Printed edition:

ISBN:

9781592592456

Access Restriction:

Restricted for use by site license.