Rice Protocols / edited by Yinong Yang.

Format:

Book

Contributor:

Yang, Yinong, 1962- editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 956.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 956

Language:

English

Subjects (All):

Life sciences.

Botany.

Plant genetics.

Life Sciences.

Plant Sciences.

Plant Genetics & Genomics.

Local Subjects:

Life Sciences.

Plant Sciences.

Plant Genetics & Genomics.

Physical Description:

1 online resource (XII, 335 pages) : 43 illustrations, 7 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.

System Details:

text file PDF

Summary:

With the completion of a finished rice genome sequence, increasing efforts have focused on functional characterization of rice genes, elucidation of the underlying mechanisms involved in major agronomic traits (e.g., high yield, grain quality, abiotic stress tolerance, and disease resistance), and the subsequent translation of genomic knowledge into agricultural productivity via molecular breeding and improved cultural practice. To meet increasing interest in this field, Rice Protocols has been compiled to provide a series of core techniques and approaches commonly used in studying rice molecular biology and functional genomics. These approaches include genetic and molecular techniques such as artificial hybridization, fluorescence in situ hybridization, generation and characterization of chemical and T-DNA insertional mutants, quantitative trait loci (QTLs) analysis and map-based cloning, site-specific transgene integration, and artificial microRNA-mediated gene silencing, along with a variety of "omics" techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Rice Protocols will prove useful for both beginners and experienced researchers whether they are molecular biologists who want to study rice plants or rice researchers who are interested in learning molecular techniques.

Contents:

Rice Artificial Hybridization for Genetic Analysis

Fluorescence In Situ Hybridization Techniques for Cytogenetic and Genomic Analyses

Generation of Rice Mutants by Chemical Mutagenesis

TILLING and Ecotilling for Rice

Functional Characterization of Rice Genes Using a Gene-Indexed T-DNA Insertional Mutant Population

QTL Analysis and Map-Based Cloning of Salt Tolerance Gene in Rice

Site-Specific Gene Integration in Rice

Transcriptome Profiling Analysis Using Rice Oligonucleotide Microarrays

Cloning of Small RNAs for the Discovery of Novel MicroRNAs in Rice

Global Identification of Small RNA Targets in Rice by Sequencing Sliced Ends of Messenger RNAs

Artificial MicroRNAs for Specific Gene Silencing in Rice

Rice Proteomic Analysis: Sample Preparation to Protein Identification

Quantification of Jasmonic and Salicylic Acids in Rice Seedling Leaves

Analysis of Insect-Induced Volatiles from Rice

Phenotypic and Physiological Evaluation for Drought and Salinity Stress Responses in Rice

Phenotypic, Physiological, and Molecular Evaluation of Rice Chilling Stress Response at the Vegetative Stage

Analysis of Rice Root Hair Morphology Using Cryo-Scanning Electron Microscopy

Inoculation and Virulence Assay for Bacterial Blight and Bacterial Leaf Streak of Rice

Inoculation and Scoring Methods for Rice Sheath Blight Disease

Molecular Approaches to Improve Rice Abiotic Stress Tolerance

Molecular Strategies to Improve Rice Disease Resistance

Molecular Strategies to Engineer Transgenic Rice Seed Compartments for Large-Scale Production of Plant-Made Pharmaceuticals

Appendix: Genomic and Bioinformatic Resources for Rice Research.

Other Format:

Printed edition:

ISBN:

9781627031943

Access Restriction:

Restricted for use by site license.