Protein Phosphatase Protocols / edited by John W. Ludlow.

Format:

Book

Contributor:

Ludlow, John W., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 93.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 93

Language:

English

Subjects (All):

Life sciences.

Biochemistry.

Life Sciences.

Biochemistry, general.

Local Subjects:

Life Sciences.

Biochemistry, general.

Physical Description:

1 online resource (XIII, 316 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1998.

System Details:

text file PDF

Summary:

In Protein Phosphatase Protocols, John Ludlow assembles a collection of cutting-edge techniques for investigating the structure and function of protein serine/threonine phosphatases. These methods are designed to enable the investigator to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources. Written by leading researchers with extensive practice in their use, each method is time-tested and sufficiently detailed to ensure reproducibility for both new and established investigators. The book also includes a discussion of the functional significance of this group of enzymes with respect to cell growth and development. Protein Phosphatase Protocols offers researchers the single best collection of step-by-step laboratory protocols now available for these important enzymes. Because of their easy reproducibility, these methods will greatly facilitate research in the area, and thus are certain to become indispensable for all those working to unlock the structure and function of the protein serine/threonine phosphatases.

Contents:

Prokaryotic Protein-Serine/Threonine Phosphatases

Protein Phosphatase Type 1 and Type 2A Assays

Analyzing Gene Expression with the Use of Serine/Threonine Phosphatase Inhibitors

Inhibitor-1, a Regulator of Protein Phosphatase 1 Function

I1 PP2A and I2 PP2A

Control of PP1 Activity Through Phosphorylation by Cyclin-Dependent Kinases

Regulation of Neuronal PP1 and PP2A During Development

PTPA Regulating PP2A as a Dual Specificity Phosphatase

Microinjection and Immunological Methods in the Analysis of Type 1 and 2A Protein Phosphatases from Mammalian Cells

Use of Immunocomplexed Substrate for Detecting PP1 Activity

The Biochemical Identification and Characterization of New Species of Protein Phosphatase 1

The Relationship Between Insulin Signaling and Protein Phosphatase 1 Activation

Analysis of the Isoforms of Protein Phosphatase 1 (PP1) Isoforms with Polyclonal Peptide Antibodies

Expression of Mouse Protein Phosphatase 2C in Eschericia coli and COS 7 Cells

Expression of Functional Protein Phosphatase 1 Catalytic Subunit in E. coli

Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana

Separation of Protein Phosphatase Type 2C Isozymes by Chromatography on Blue Sepharose

Chromatographic Isolation of PP2A from Limulus Lateral Eyes

Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C from the Yeast Saccharomyces cerevisiae

Molecular Cloning of Protein Phosphatase Type 2C Isoforms from Retinal cDNA

Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits Using the Yeast Two-Hybrid Assay

Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-Hybrid Method

Protein Phosphatase 2A Regulatory Subunits

Synthetic Lethal Screening in Protein Phosphatase Pathways

The Search for the Biological Function of Novel Yeast Ser/Thr Phosphatases.

Other Format:

Printed edition:

ISBN:

9781592592678

Access Restriction:

Restricted for use by site license.