Neural Tracing Methods : Tracing Neurons and Their Connections / edited by Benjamin R. Arenkiel.

Format:

Book

Contributor:

Arenkiel, Benjamin R., editor.

SpringerLink (Online service)

Series:

Neuromethods 0893-2336 ; 92.

Springer Protocols (Springer-12345)

Neuromethods, 0893-2336 ; 92

Language:

English

Subjects (All):

Neurosciences.

Neurobiology.

Local Subjects:

Neurosciences.

Neurobiology.

Physical Description:

1 online resource (XII, 278 pages) : 52 illustrations, 47 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2015.

System Details:

text file PDF

Summary:

This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.

Contents:

A Survey of Current Neuroanatomical Tracing Techniques.- Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry

Retrograde Tract-Tracing 'Plus': Adding Extra Value to Retrogradely-Traced Neurons

Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice

Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics

Practical Methods for In Vivo Cortical Physiology with 2-Photon Microscopy and Bulk Loading of Fluorescent Calcium Indicator Dyes

Optogenetic Dissection of Neural Circuit Function in Behaving Animals

Remote Control of Neural Activity Using Chemical Genetics

Generation of BAC Transgenic Mice for Functional Analysis of Neural Circuits

Engineered Rabies Virus for Transsynaptic Circuit Tracing

Genetic Labeling of Synapses

Genetic Pathways to Circuit Understanding in Drosophila.

Other Format:

Printed edition:

ISBN:

9781493919635

Access Restriction:

Restricted for use by site license.