Sexually Transmitted Diseases : Methods and Protocols / edited by Rosanna W. Peeling, P. Frederick Sparling.

Format:

Book

Contributor:

Peeling, Rosanna W., editor.

Sparling, P. Frederick, editor.

SpringerLink (Online service)

Series:

Methods in molecular medicine 1543-1894 ; 20.

Springer Protocols (Springer-12345)

Methods in Molecular Medicine™, 1543-1894 ; 20

Language:

English

Subjects (All):

Medicine.

Infectious diseases.

Medicine & Public Health.

Infectious Diseases.

Local Subjects:

Medicine & Public Health.

Infectious Diseases.

Physical Description:

1 online resource (X, 244 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 1999.

System Details:

text file PDF

Summary:

In Sexually Transmitted Diseases: Methods and Protocols, Rosanna Peeling and P. Frederick Sparling have created a comprehensive collection of cutting-edge molecular protocols for the laboratory diagnosis and study of sexually transmitted diseases, including HIV. The methods-each crafted by a leading expert for comprehensiveness and genuine laboratory utility-cover the major sexually transmitted pathogens, including Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilius ducreyl, Treponema pallidum, and herpes simplex. Additional molecular techniques are included for detecting the genital mycoplasmas, hepatitis B virus, HIV-1, human papillomavirus. This book will significantly enhance our ability to detect sexually transmitted infections and to conduct research to further our understanding of sexually transmitted diseases. Eminently practical and up-to-date, Sexually Transmitted Disease Protocols provides a comprehensive collection of reliable molecular methods for STD diagnosis, research, and patient management.

Contents:

Overview

The Impact of Molecular Technology on STD Control

Molecular Techniques for the Detection of Sexually Transmitted Pathogens

Neisseria gonorrhoeae

Molecular Diagnosis of Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR,TMA, and Q-? Replicase

Haemophilus ducreyi Detection by PCR

Detection of Treponema pallidum, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR

Detection of Genital Mycoplasmas by PCR

Hepatitis B Virus

Molecular Diagnosis of HIV-1 by PCR

Research Techniques for Epidemiology and the Management of STDs

Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR

Genotyping Chlamydia trachomatis by PCR

Human Papillomavirus Detection by PCR and Typing by Dot-Blot

Quantitation of HIV-1 RNA in Dried Plasma Spots (DPS)

Collection and Processing of Seminal Plasma for the Quantitation of HIV-1 RNA by NASBA and RT-PCR

Molecular Technology for STD Diagnosis and Research: the New Challenges

Molecular Techniques for HIV and STDs.

Other Format:

Printed edition:

ISBN:

9781592592128

Access Restriction:

Restricted for use by site license.