2 options
Pharmacogenomics : Methods and Protocols / edited by Federico Innocenti.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 311.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 311
- Language:
- English
- Subjects (All):
- Pharmacy.
- Local Subjects:
- Pharmacy.
- Physical Description:
- 1 online resource (X, 214 pages).
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press, 2005.
- System Details:
- text file PDF
- Summary:
- Pharmacogenomics holds the promise of drugs tailor-made to an individual's genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for today's preclinical and clinical studies.
- Contents:
- History and Overview
- Pharmacogenomics
- Functional Analysis of Gene Variation
- Transfection Assays With Allele-Specific Constructs
- Snapshot of the Allele-Specific Variation in Human Gene Expression
- Genome-Wide Analysis of Allele-Specific Gene Expression Using Oligo Microarrays
- HaploChIP
- Genotyping Techniques
- Aspects Influencing Genotyping Method Selection
- Denaturing High-Performance Liquid Chromatography for Mutation Detection and Genotyping
- Pyrosequencing of Clinically Relevant Polymorphisms
- Kinetic Fluorescence-Quenching Detection Assay for Allele Frequency Estimation
- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
- Fluorescence-Based Fragment Size Analysis
- Single-Nucleotide Polymorphism Genotyping in DNA Pools
- TaqMan Genotyping of Insertion/Deletion Polymorphisms
- Management of Pharmacogenomic Information
- PharmGKB
- Systems for the Management of Pharmacogenomic Information.
- Other Format:
- Printed edition:
- ISBN:
- 9781592599578
- Access Restriction:
- Restricted for use by site license.
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.