Cancer Gene Profiling : Methods and Protocols / edited by Robert Grützmann, Christian Pilarsky.

Format:

Book

Contributor:

Grützmann, Robert, editor.

Pilarsky, Christian, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 576.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 576

Language:

English

Subjects (All):

Medicine.

Cancer--Research.

Cancer.

Gene expression.

Oncology.

Medicine & Public Health.

Cancer Research.

Gene Expression.

Local Subjects:

Medicine & Public Health.

Oncology.

Cancer Research.

Gene Expression.

Physical Description:

1 online resource (XII, 452 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2010.

System Details:

text file PDF

Summary:

Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research.

Contents:

Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Support of Biomedical Research

Manual Microdissection

Laser Microdissection

Tissue Microarrays

A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function

Mining Expressed Sequence Tag (EST) Libraries for Cancer-Associated Genes

Automated Fluorescent Differential Display for Cancer Gene Profiling

Manual Microdissection Combined with Antisense RNA-LongSAGE for the Analysis of Limited Cell Numbers

Quantitative DNA Methylation Profiling on a High-Density Oligonucleotide Microarray

Single-Nucleotide Polymorphism (SNP) Analysis to Associate Cancer Risk

Application of Proteomics in Cancer Gene Profiling: Two-Dimensional Difference in Gel Electrophoresis (2D-DIGE)

Search for and Identification of Novel Tumor-Associated Autoantigens

Integrative Oncogenomic Analysis of Microarray Data in Hematologic Malignancies

Cancer Gene Profiling in Pancreatic Cancer

Cancer Gene Profiling in Prostate Cancer

Cancer Gene Profiling for Response Prediction

The EGFR Pathway as an Example for Genotype: Phenotype Correlation in Tumor Genes

Quantitation of CD39 Gene Expression in Pancreatic Tissue by Real-Time Polymerase Chain Reaction

Functional Profiling Methods in Cancer

Calibration of Microarray Gene-Expression Data

Meta-analysis of Cancer Gene-Profiling Data

Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment.

Other Format:

Printed edition:

ISBN:

9781597455459

Access Restriction:

Restricted for use by site license.