Yeast Functional Genomics : Methods and Protocols / edited by Frédéric Devaux.

Format:

Book

Contributor:

Devaux, Frédéric, 1972- editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1361.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 1361

Language:

English

Subjects (All):

Life sciences.

Gene expression.

Microbiology.

Life Sciences.

Gene Expression.

Local Subjects:

Life Sciences.

Microbiology.

Gene Expression.

Physical Description:

1 online resource (XI, 406 pages) : 77 illustrations, 51 illustrations in color.

Edition:

First edition 2016.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2016.

System Details:

text file PDF

Summary:

This volume provides a collection of protocols for the study of DNA-DNA contact maps, replication profiles, transcription rates, RNA secondary structures, protein-RNA interactions, ribosome profiling and quantitative proteomes and metabolomes. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Yeast Functional Genomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species

Enhancing Structural Annotation of Yeast Genomes with RNA-Seq Data

Pathogen Gene Expression Profiling During Infection Using a Nanostring Ncounter Platform

Comparative Transcriptomics in Yeasts

Mapping the Transcriptome-Wide Landscape of RBP Binding sites using gPAR-CLIP-seq: Experimental Procedures

Mapping the Transcriptome-wide Landscape of RBP Binding Sites using gPAR-CLIP-seq: Bioinformatic Analysis

Translation Analysis at the Genome Scale by Ribosome Profiling

Biotin-Genomic Run-on (Bio-GRO): A High-Resolution Method for the Analysis of Nascent Transcription in Yeast

Genome-wide Probing of RNA Structures in Vitro Using Nucleases and Deep Sequencing

Genome-wide Chromatin Immunoprecipitation in Candida albicans and other Yeasts

ChIPseq in Yeast Species: from Chromatin Immumoprecipitation to High-Throughput Sequencing and Bioinformatics Data Analyses

Systematic Determination of Transcription factor DNA-binding Specificities in Yeast

Generation and Analysis of Chromosomal Contact Maps of Yeast Species

A Versatile Procedure to Generate Genome-Wide Spatio-Temporal Program of Replication in Yeast Species

Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular Complexes in the Yeast S. cerevisiae

Label-Free Quantitative Proteomics in Yeast

Profiling of Yeast Lipids by Shotgun Lipidomics

Identification of Links between Cellular Pathways by Genetic Interaction Mapping (GIM)

On The Mapping of Epistatic Genetic Interactions in Natural Isolates: Combining Classical Genetics and Genomics

Experimental Evolution and Resequencing Analysis of Yeast

Reconstruction and Analysis of the Evolution of Modular Transcriptional Regulatory Programs using Arboretum

Predicting Gene and Genomic Regulation in Saccharomyces cerevisiae, using the YEASTRACT Database: A Step By Step Guided Analysis.

Other Format:

Printed edition:

ISBN:

9781493930791

Access Restriction:

Restricted for use by site license.