Cystic Fibrosis : Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis / edited by Margarida D. Amaral, Karl Kunzelmann.

Format:

Book

Contributor:

Amaral, Margarida D., editor.

Kunzelmann, Karl, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 742.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 742

Language:

English

Subjects (All):

Medicine.

Human genetics.

Diagnosis, Laboratory.

Molecular biology.

Biomedicine.

Molecular Medicine.

Human Genetics.

Laboratory Medicine.

Local Subjects:

Biomedicine.

Molecular Medicine.

Human Genetics.

Laboratory Medicine.

Physical Description:

1 online resource (XIV, 384 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature. Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis. Volume II: Methods and Resources to Understand Cystic Fibrosis focuses on pathophysiology, Omics approaches, and a variety of key resources recently made available for CF research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it.

Contents:

Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology

Imaging CFTR Protein Localization in Cultured Cells and Tissues

CFTR Regulation of Epithelial Sodium Channel

Methods for Evaluating Inflammation in Cystic Fibrosis

Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures

Measurement of Fluid Secretion from Intact Airway Submucosal Glands

Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia

Identification and Quantification of Mucin Expression

Methods to Classify Bacterial Pathogens in Cystic Fibrosis

Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells

Introduction to Part II: Omics in the Biology of Cystic Fibrosis

Microarray mRNA Expression Profiling to Study Cystic Fibrosis

Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)

Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis

Functional Genomics Assays to Study CFTR Traffic and ENaC Function

New Lipidomic Approaches in Cystic Fibrosis

Introduction to Part III: Resources for CFTR Research

Primary Epithelial Cell Models for Cystic Fibrosis Research

Comparative Biology of Cystic Fibrosis Animal Models

CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction

Evaluation of the Disease Liability of CFTR Variants.

Other Format:

Printed edition:

ISBN:

9781617791208

Access Restriction:

Restricted for use by site license.