Posttranslational Modifications of Proteins : Tools for Functional Proteomics / edited by Christoph Kannicht.

Format:

Book

Contributor:

Kannicht, Christoph, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 194.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 194

Language:

English

Subjects (All):

Life sciences.

Cytology.

Life Sciences.

Cell Biology.

Local Subjects:

Life Sciences.

Cell Biology.

Physical Description:

1 online resource (XI, 322 pages) : 19 illustrations.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2002.

System Details:

text file PDF

Summary:

The majority of all proteins undergo posttranslational modifications that significantly alter their physical and chemical properties, including their folding and conformation distribution, their stability, and, consequently, their activity and function. In Posttranslational Modifications of Proteins: Tools for Functional Proteomics, Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the most important of these modifications, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation. Comprehensive and state-of-the-art, Posttranslational Modifications of Proteins: Tools for Functional Proteomics serves as a highly practical guide for all investigators of protein structure-function relationships not only in chemical and pharmaceutical research, but also throughout the rapidly growing field of functional proteomics.

Contents:

Assignment of Disulfide Bonds in Proteins by Chemical Cleavage and Peptide Mapping by Mass Spectrometry

Carbohydrate Composition Analysis of Glycoproteins Using Highly Sensitive Fluorescence Detection Methods

Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB)

Separation of N-Glycans by HPLC

Enzymatic Sequence Analysis of N-Glycans

Immunological Detection of O-GlcNAc

Analysis of O-Glycosylation

Characterization of Site-Specific Glycosylation

Monitoring Glycosylation of Therapeutic Glycoproteins for Consistency Using Highly Fluorescent Anthranilic Acid

Metabolic Labeling and Structural Analysis of Glycosylphosphatidylinositols from Parasitic Protozoa

Analysis of S-Acylation of Proteins

Immunoblotting Methods for the Study of Protein Ubiquitination

Analysis of Methylation and Acetylation in E. coli Ribosomal Proteins

Identification of In Vivo Protein Phosphorylation Sites with Mass Spectrometry

Analysis of Tyrosine-O-Sulfation

?-Amidated Peptides: Approaches for Analysis

?-Glutamate and p-Hydroxyaspartate in Proteins

Detection of isoAspartate Residues as a Posttranslational Modification of Proteins and Peptides

Lysine Hydroxylation and Crosslinking of Collagen

Heterologous Expression in Endocrine Cells for Analysis of Posttranslational Modifications

2D-Electrophoresis: Detection of Glycosylation and Influence on Spot Pattern.

Other Format:

Printed edition:

ISBN:

9781592591817

Access Restriction:

Restricted for use by site license.