Cardiac Gene Expression : Methods and Protocols / edited by Jun Zhang, Gregg Rokosh.

Format:

Book

Contributor:

Zhang, Jun, 1968- editor.

Rokosh, Gregg, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 366.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, 1064-3745 ; 366

Language:

English

Subjects (All):

Life sciences.

Human genetics.

Cardiology.

Biochemistry.

Bioinformatics.

Life Sciences.

Biochemistry, general.

Human Genetics.

Local Subjects:

Life Sciences.

Biochemistry, general.

Human Genetics.

Cardiology.

Bioinformatics.

Physical Description:

1 online resource (XII, 366 pages) : 66 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2007.

System Details:

text file PDF

Summary:

Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.

Contents:

Cardiac Gene Expression

Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells

Expression Profiling Using Affymetrix GeneChip® Probe Arrays

Serial Analysis of Gene Expression (SAGE)

Functional Genomics by cDNA Subtractive Hybridization

Statistical Methods in Cardiac Gene Expression Profiling

Cardiac Gene Regulation

Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR)

Quantitative (Real-Time) RT-PCR in Cardiovascular Research

RNase Protection Assay for Quantifying Gene Expression Levels

In Situ Hybridization

Characterization of cis-Regulatory Elements and Transcription Factor Binding

Mapping Transcriptional Start Sites and In Silico DNA Footprinting

Characterization of Cardiac Gene Promoter Activity

In Silico Assessment of Regulatory cis-Elements and Gene Regulation

Comparative Genomics

Developing Computational Resources in Cardiac Gene Expression

Cardiac Single Nucleotide Polymorphisms

In Silico Analysis of SNPs and Other High-Throughput Data

Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS

Gene Overexpression and Targeting in the Myocardium

Conditional Targeting

Cardiomyocyte Preparation, Culture, and Gene Transfer

Adeno-Associated Viral Vector-Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts

Lentivirus-Mediated Gene Expression.

Other Format:

Printed edition:

ISBN:

9781597450300

Access Restriction:

Restricted for use by site license.