Molecular Toxicology Protocols / edited by Phouthone Keohavong, Stephen G. Grant.

Format:

Book

Contributor:

Keohavong, Phouthone, editor.

Grant, Stephen G., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 291.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 291

Language:

English

Subjects (All):

Medicine.

Pharmacology.

Biochemistry.

Biomedicine.

Pharmacology/Toxicology.

Biochemistry, general.

Local Subjects:

Biomedicine.

Pharmacology/Toxicology.

Biochemistry, general.

Physical Description:

1 online resource (XIV, 489 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2005.

System Details:

text file PDF

Summary:

New state-of-the-art molecular techniques promise to transform the field of genetic toxicology by making it possible to directly detect genotoxic exposures and their consequences in humans, to identify the agent(s) involved, and to clinically manage the exposed population. In Molecular Toxicology Protocols, researchers from prominent universities and cancer centers around the world describe in detail their best techniques for analyzing genotoxic exposure and the resulting biological effects, including intermediate biomarkers such as DNA and chromosomal damage, mutations in reporter and oncogenes, and the earliest possible detection of cancer. The authors emphasize analytical methods specifically developed for use in human populations and in cancer patients, or in other in vivo systems such as transgenic mice. Among the applications detailed are the analysis of interactions of chemical and physical agents with cellular macromolecules, especially DNA, the assessment of medically relevant toxicity, and the individualized characterization of genetic damage and repair. There are also methods to assess and characterize the modulation of this damage and repair through individual differences in specific and genome-wide gene expression, including metabolic profiling and apoptotic capacity. These methods mark a shift in emphasis from studies of the agents themselves to the exposed population, and from studies of small populations with significant known exposures to a single agent, to studies of common diseases, such as breast cancer, caused by normal levels of generalized genotoxic exposure. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Molecular Toxicology Protocols offers a gold-standard collection of cutting-edge techniques designed to investigate a broad range of exposures-endogenous, accidental, medical, environmental, and occupational-and their role in human carcinogenesis and other diseases of aging.

Contents:

Analysis of DNA Adducts

32P-Postlabeling Analysis of DNA Adducts

Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot

DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry

Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles

Analysis of DNA Strand Cleavage at Abasic Sites

Detection of Chromosomal and Genome-Wide Damage

Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes

Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk

Flow Cytometric Analysis of Micronuclei in Erythrocytes

The Comet Assay

Computerized Image Analysis Software for the Comet Assay

The Comet-FISH Technique

DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis

Detection and Characterization of Surrogate Gene Mutation

Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes

Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems

Methods for Detecting Somatic Mutations In Vitro

Molecular Analysis of Mutations in the Human HPRT Gene

Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes

The GPA In Vivo Somatic Mutation Assay

Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR

Detection and Characterization of Cancer Gene Mutation

Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis

Analysis of K-RAS and P53 Mutations in Sputum Samples

Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution

Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection

Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions

Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR

Analysis of DNA Repair Mechanisms

Microsatellite Instability

Unscheduled DNA Synthesis

Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation

An Immunoassay for Measuring Repair of Ultraviolet Photoproducts

Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells

Measuring Recombination Proficiency in Mouse Embryonic Stem Cells

Array Technologies

Strategies for Measurement of Biotransformation Enzyme Gene Expression

Genotyping Technologies

TaqMan® Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material

Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression

Apoptosis

Quantification of Selective Phosphatidylserine Oxidation During Apoptosis

Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron

Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.

Other Format:

Printed edition:

ISBN:

9781592598403

Access Restriction:

Restricted for use by site license.