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Viral Applications of Green Fluorescent Protein : Methods and Protocols / edited by Barry W. Hicks.
Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in molecular biology 1064-3745 ; 515.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology™, 1064-3745 ; 515
- Language:
- English
- Subjects (All):
- Life sciences.
- Diagnosis, Laboratory.
- Proteins.
- Life Sciences.
- Protein Science.
- Laboratory Medicine.
- Local Subjects:
- Life Sciences.
- Protein Science.
- Laboratory Medicine.
- Physical Description:
- 1 online resource (315 pages) : 81 illustrations.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press : Imprint: Humana Press, 2009.
- System Details:
- text file PDF
- Summary:
- Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.
- Contents:
- Using Fluorescent Proteins to Study Poxvirus Morphogenesis
- Molecular Imaging of Human Embryonic Stem Cells
- Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection
- Dual-Color Imaging of Tumor Angiogenesis
- Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System
- Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System
- Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes
- Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry
- Production of Multicistronic HIV-1 Based Lentiviral Vectors
- Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps
- Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs
- GFP-Lentiviral Vectors Targeting for NeuroAIDS
- Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery
- Selective Protein Expression Within the CNS Using Hybrid Lentivirus
- Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein
- The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication
- Modified Bovine Herpesvirus 1 for Protein Secretion
- Identification of Viral Peptide Fragments for Vaccine Development
- Use of GFP to Investigate Expression of Plant-Derived Vaccines
- Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions
- Sendai Virus for Cancer Immunotherapy
- Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells
- Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity
- Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells.
- Other Format:
- Printed edition:
- ISBN:
- 9781597455596
- Access Restriction:
- Restricted for use by site license.
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