Seed Dormancy : Methods and Protocols / edited by Allison R. Kermode.

Format:

Book

Contributor:

Kermode, Allison R., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 773.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 773

Language:

English

Subjects (All):

Life sciences.

Botany.

Plant physiology.

Life Sciences.

Plant Sciences.

Plant Physiology.

Local Subjects:

Life Sciences.

Plant Sciences.

Plant Physiology.

Physical Description:

1 online resource (XVI, 415 pages) : 85 illustrations, 1 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2011.

System Details:

text file PDF

Summary:

Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.

Contents:

Challenges Facing the Forest Industry in Relation to Seed Dormancy and Seed Quality

Challenges Facing Seed Banks and Agriculture in Relation to Seed Quality

Standardizing Seed Dormancy Research

Eyeing Emergence: Modified Treatments for Terminating Dormancy of Conifer Seeds

Production of Seed Samples for the Effective Molecular Analysis of Dormancy Cycling in Arabidopsis

Approaches to the Identification of ABAR as an Abscisic Acid Receptor

Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies

In Vitro Assay for ABA 8'-Hydroxylase: Implications for Improved Assays for Cytochrome P450 Enzymes

Functional Analysis of Abscisic Acid 8'-Hydroxylase

Isolation of Genetic Material from Arabidopsis Seeds

Identification and Characterization of Quantitative Trait Loci (QTL) that Control Dormancy in Arabidopsis

Identification of Seed Dormancy Mutants by Activation Tagging

Isolation of MicroRNAs that Regulate Seed Dormancy and Germination

Proteomics and Post-Translational Proteomics of Seed Dormancy and Germination

Chromatin Dynamics during Seed Dormancy

Emerging Mass Spectrometry-Based Technologies for Analyses of Chromatin Changes: Analysis of Histones and Histone Modifications

In Vivo Nuclear Magnetic Resonance (NMR) Metabolite Profiling in Plant Seeds

In Vivo 1H NMR Microimaging during Seed Imbibition, Germination, and Early Growth

Tissue Printing Methods for Localization of RNA and Proteins that Control Seed Dormancy and Germination

Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination

Protein Damage and Repair Controlling Seed Vigor and Longevity

Nitric Oxide Methods in Seed Biology

Seed Bioinformatics.

Other Format:

Printed edition:

ISBN:

9781617792311

Access Restriction:

Restricted for use by site license.