Plant Fungal Pathogens : Methods and Protocols / edited by Melvin D. Bolton, Bart P.H.J. Thomma.

Format:

Book

Contributor:

Bolton, Melvin D. (Melvin Dennis), 1977- editor.

Thomma, Bart P. H. J., editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 835.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 835

Language:

English

Subjects (All):

Life sciences.

Botany.

Plant diseases.

Life Sciences.

Plant Sciences.

Plant Pathology.

Local Subjects:

Life Sciences.

Plant Sciences.

Plant Pathology.

Physical Description:

1 online resource (XVI, 648 pages) : 138 illustrations, 72 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2012.

System Details:

text file PDF

Summary:

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor. In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.

Contents:

Fungal ABC Transporter Deletion and Localization Analysis

Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated Transformation

Activity-based Protein Profiling of Infected Plants

The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves

Proteomic Techniques for Plant-Fungal Interactions

Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway®-Compatible Binary Potato Virus X-Expression Vector

Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens

Real-time PCR for the Quantification of Fungi in planta

Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi

Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates

Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors

Characterization of Plant - Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens

Heterologous Production of Fungal Effectors in Pichia pastoris

The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta

Metabolomics Protocols for Filamentous Fungi

Targeted Gene Replacement in Fungi Using a Split-Marker Approach

Massively Parallel Sequencing Technology in Pathogenic Microbes

Confocal Microscopy in Plant Pathogen Interactions

The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data

Population Biology of Fungal Plant Pathogens

Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens

In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi

Fungicide Resistance Assays for Fungal Plant Pathogens

Identification of Lipid-binding Effectors

In silico Identification and Characterization of Effector Catalogs

Horizontal Transfer of Supernumerary Chromosomes in Fungi

The Induction of Mycotoxins by Trichothecene Producing Fusarium Species

Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics

Assessment of Autophagosome Formation by Transmission Electron Microscopy (TEM)

Fungal Plant Pathogen Detection in Plant and Soil Samples using DNA Macroarrays

Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors

A Yeast-Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and their Plant Hosts

Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes

Multi-gene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens

MAP Kinase Phosphorylation and cAMP Assessment in Fungi

A One-step Affinity-purification Protocol to Purify NB-LRR Immune Receptors from Plants that Mediate Resistance to Fungal Pathogens

Karyotyping Methods for Fungi

Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique

Gene Cloning using Degenerate Primers and Genome Walking

Construction of Hairpin RNA Expressing Vectors for RNA-mediated Gene Silencing in Fungi

An Unbiased Method for the Quantitation of Disease Phenotypes using a Custom-built Macro Plugin for the Program ImageJ.

Other Format:

Printed edition:

ISBN:

9781617795015

Access Restriction:

Restricted for use by site license.